Penehyclidine hydrochloride postconditioning on lipopolysaccharide-induced acute lung injury by inhibition of inflammatory factors in a rodent model
| Autor: | Li Liu, Gang-Ming Wu, Changhe Ren, Li-Qun Mo, Ye Chen, Jun Zhou, Min Mou |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Lipopolysaccharides
Male Quinuclidines Acute Lung Injury Drug Evaluation Preclinical Lung injury Pharmacology Rats Sprague-Dawley Sepsis Fraction of inspired oxygen medicine Animals Lung Peroxidase medicine.diagnostic_test biology Interleukin-6 Pulmonary Gas Exchange Tumor Necrosis Factor-alpha business.industry NF-kappa B respiratory system medicine.disease respiratory tract diseases Disease Models Animal Bronchoalveolar lavage medicine.anatomical_structure Myeloperoxidase Anesthesia biology.protein Arterial blood Surgery Tumor necrosis factor alpha business Bronchoalveolar Lavage Fluid |
| Zdroj: | Journal of Surgical Research. 195:219-227 |
| ISSN: | 0022-4804 |
| DOI: | 10.1016/j.jss.2014.12.018 |
| Popis: | Background Sepsis is associated with acute lung injury (ALI) and high mortality. The aim of this study was to investigate the effects of different doses of penehyclidine hydrochloride (PHC) postconditioning on ALI induced by sepsis in a rat model. Methods A rat model of ALI was induced by intravenous injection of lipopolysaccharide (LPS). The different doses of PHC were administrated intravenously at 30 min after LPS administration (low dose, 0.3 mg/kg; medium dose, 1.0 mg/kg, and high dose, 3.0 mg/kg). After 6 h, arterial blood samples were obtained for blood gas analyses. Meanwhile, lung tissue was harvested and lung injury was assessed by the histopathologic changes (hematoxylin and eosin staining) and wet-to-dry lung weight ratio. The tumor necrosis factor-α and interleukin-6 levels in bronchoalveolar lavage fluid, as well as the nuclear factor-kappa B protein expressions, and the myeloperoxidase activities in lung tissues were measured by immunohistochemistry or enzyme-linked immunosorbent assay, respectively. Results LPS-induced severe lung injury evidenced by increased pathologic scores and lung wet-to-dry weight ratio, which was accompanied by increases in the expression of pulmonary nuclear factor-kappa B protein and the activity of pulmonary myeloperoxidase and the levels of interleukin-6 and tumor necrosis factor-α in bronchoalveolar lavage fluid. The arterial oxygen tension (PaO2), pH, and the PaO2/fraction of inspired oxygen ratio (PaO2/FiO2) decreased significantly and the carbon dioxide tension (PaCO2) increased notedly after an LPS injection. All doses of PHC could significantly ameliorate lung injury and improve the previously mentioned variables (P |
| Databáze: | OpenAIRE |
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