Functional Detection of TNF Receptor Family Members by Affinity-Labeled Ligands
| Autor: | Xinyu Liu, Joshua G. Liang, Xiaojun Liu, Anliang Huang, Yang Xu, Peng Liang, Lingmo Chang, Hong Zhou |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Cell type Science CHO Cells Ligands Chromatography Affinity Receptors Tumor Necrosis Factor Article 03 medical and health sciences Mice 0302 clinical medicine Cricetulus In vivo Animals Humans Receptor Author Correction Multidisciplinary biology Protein Stability Chinese hamster ovary cell Affinity Labels biology.organism_classification Alkaline Phosphatase HCT116 Cells Molecular biology In vitro 030104 developmental biology 030220 oncology & carcinogenesis biology.protein Cancer research Alkaline phosphatase Medicine Thermodynamics Antibody |
| Zdroj: | Scientific Reports Scientific Reports, Vol 7, Iss 1, Pp 1-7 (2017) |
| ISSN: | 2045-2322 |
| Popis: | Aberrant expression of TNF family of cytokines has been linked to human diseases, and biologics targeting their signaling have become the best selling drugs globally. However, functional detection with labeled ligands for accurate detection of TNFR family of receptor-expressing target tissues or cell types remains to be developed. Here we show that TNF receptor family members are heat-stable and can be recognized both in vitro and in vivo by their ligands labeled with alkaline phosphatase. Such an approach may be used in lieu of antibodies for the identification of the cell types involved in receptor signaling during disease onset and progression. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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