Generation of Cdx2-mCherry knock-in murine ES cell line to model trophectoderm and intestinal lineage differentiation
| Autor: | Hanuman T. Kale, P. Chandra Shekar, Debabrata Jana |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Biology Cell Line 03 medical and health sciences Mice 0302 clinical medicine Gene knockin Animals CDX2 Transcription Factor Cell Lineage CDX2 Transcription factor lcsh:QH301-705.5 Embryonic Stem Cells Regulation of gene expression Lateral plate mesoderm Gene Expression Regulation Developmental Cell Differentiation Cell Biology General Medicine Intestinal epithelium digestive system diseases Cell biology Intestines 030104 developmental biology lcsh:Biology (General) embryonic structures Homeobox mCherry 030217 neurology & neurosurgery Developmental Biology |
| Zdroj: | Stem Cell Research, Vol 39, Iss, Pp-(2019) |
| ISSN: | 1873-5061 |
| Popis: | Caudal-type homeobox 2 (.Cdx2) transcription factor is an essential regulator of differentiation to the intestinal epithelium, somatic mesoderm and trophectoderm function in the mouse. However, the regulation of Cdx2 in these processes is poorly understood. Separation of viable Cdx2 expressing cells during differentiation for downstream experiments is not possible due to its nuclear localization, limiting experimental possibilities and studying Cdx2 regulation. Here, we report generation of a Cdx2-mCherry knock-in reporter mouse embryonic stem cell line (TCMC), for modeling and studying in vitro differentiation of mESCs to intestinal epithelia, somatic mesoderm, and trophectoderm. |
| Databáze: | OpenAIRE |
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