Multinational evaluation of the BioFire® FilmArray® Pneumonia plus Panel as compared to standard of care testing

Autor: Ginocchio, C. C., Garcia-Mondragon, C., Mauerhofer, B., Rindlisbacher, C., Forcelledo, L., Fernandez, J., Lienhard, R., Kerschner, H., Rossolini, G. M., Armand-Lefevre, L., D'Humieres, C., Cambau, E., Benmansour, H., Cavallo, R., Altwegg, M., Berlinger, L., Bonnet, R., Saint-Sardos, P., Meex, C., Lavigne, J. P., Leveque, N., Broutin, L., Cattoir, V., Auger, G., Pereira, H., Paitan, Y., Verroken, A., Pailhories, H., Lemarie, C., Martinetti-Lucchini, G., Frigerio Malossa, S., Sanguinetti, M., Spanu, T., Vandenesch, F., Poyart, C., Loubinoux, J., Mira, J. P., Bonacorsi, S., Cointe, A., Munoz, P., Kestler, M., Esteva, C., Queralt, X., Garcia-Rodriguez, J., Gomez, M. D., Lopez-Hontangas, J. L., Ghisetti, V., Burdino, E., Schubert, S., Mencacci, A., Allegrucci, F., Rozemeijer, W., Paternotte, N., Allard, A., M. C., Re, Ambretti, S., Skov, M., Agergaard, C. N., Subudhi, P., Wichelhaus, T. A., Egli, A., Hinic, V., Alcock, A., Banavathi, K., Tiberio, C., Ruocco, G., Atripaldi, L.
Rok vydání: 2021
Předmět:
Zdroj: European Journal of Clinical Microbiology & Infectious Diseases
and the EME Evaluation Program Collaborative, Skov, M N & Agergaard, C N 2021, ' Multinational evaluation of the BioFire® FilmArray® Pneumonia plus Panel as compared to standard of care testing ', European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology, vol. 40, no. 8, pp. 1609–1622 . https://doi.org/10.1007/s10096-021-04195-5
ISSN: 1435-4373
0934-9723
DOI: 10.1007/s10096-021-04195-5
Popis: This study compared standard of care testing (SOC) to BioFire® FilmArray® Pneumonia plus Panel (PNplus). PNplus detects 15 bacteria with semiquantitative log bin values, 7 antibiotic resistance markers, three atypical bacteria (AB), and eight viral classes directly from bronchoalveolar lavage-like specimens (BLS) and sputum-like specimens (SLS). Fifty-two laboratories from 13 European countries and Israel tested 1234 BLS and 1242 SLS with PNplus and SOC. Detection rates and number of pathogens/samples were compared for PNplus pathogens. PNplus bin values and SOC quantities were compared. Three thousand two hundred sixty-two bacteria in PNplus were detected by PNplus and/or SOC. SOC detected 57.1% compared to 95.8% for PNplus (p ≤ 0.0001). PNplus semiquantitative bin values were less than SOC, equal to SOC, or greater than SOC in 5.1%, 25.4%, and 69.6% of results, respectively. PNplus bin values were on average ≥ 1 log than SOC values (58.5% 1–2 logs; 11.0% 3–4 logs). PNplus identified 98.2% of MRSA and SOC 55.6%. SOC detected 73/103 AB (70.9%) and 134/631 viruses (21.2%). PNplus detected 93/103 AB (90.3%) and 618/631 viruses (97.9%) (p ≤ 0.0001). PNplus and SOC mean number of pathogens/samples were 1.99 and 1.44, respectively. All gram-negative resistance markers were detected. PNplus and SOC results were fully or partially concordant for 49.1% and 26.4% of specimens, respectively. PNplus was highly sensitive and detected more potential pneumonia pathogens than SOC. Semiquantification may assist in understanding pathogen significance. As PNplus generates results in approximately 1 h, PNplus has potential to direct antimicrobial therapy in near real time and improve antimicrobial stewardship and patient outcomes.
Databáze: OpenAIRE
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