Expansion of Multipotent Stem Cells from the Adult Human Brain
| Autor: | Ingunn Ramsnes, Sheryl Palmero, Wayne Murrell, Linda Paulson, Ståle Nygård, Iver A. Langmoen, Einar O. Vik-Mo, Cecilie Sandberg, Petter Brandal, Håvard K. Skjellegrind, Zanina Grieg, Mrinal Joel, Emily Palmero, John Bianco, Bernd Thiede, Biljana Stangeland |
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| Rok vydání: | 2013 |
| Předmět: |
Proteomics
Anatomy and Physiology Microarrays Cell Culture Techniques lcsh:Medicine Cell Fate Determination Transcriptomes Neurological Signaling Neural Stem Cells Molecular Cell Biology Stem Cell Niche lcsh:Science Stem cell transplantation for articular cartilage repair Neurons Multidisciplinary Stem Cells Brain Cell Differentiation Genomics Middle Aged Signaling in Selected Disciplines Neural stem cell Cell biology Adult Stem Cells Phenotype Neurology Medicine Cellular Types Stem cell Cell Division Research Article Signal Transduction Cerebral organoid Adult stem cell Adult Cell Physiology Induced Pluripotent Stem Cells Neurosurgery Clinical uses of mesenchymal stem cells Biology Neurological System Developmental Neuroscience Genome Analysis Tools Neurorehabilitation and Trauma Neurosphere Humans Staining and Labeling Dopaminergic Neurons Multipotent Stem Cells lcsh:R Computational Biology Clone Cells Gene Expression Regulation Multipotent Stem Cell Karyotyping Cellular Neuroscience Immunology Surgery lcsh:Q Molecular Neuroscience Stem Cell Lines Biomarkers Developmental Biology Neuroscience |
| Zdroj: | PLoS ONE, Vol 8, Iss 8, p e71334 (2013) PLoS ONE |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0071334 |
| Popis: | The discovery of stem cells in the adult human brain has revealed new possible scenarios for treatment of the sick or injured brain. Both clinical use of and preclinical research on human adult neural stem cells have, however, been seriously hampered by the fact that it has been impossible to passage these cells more than a very few times and with little expansion of cell numbers. Having explored a number of alternative culturing conditions we here present an efficient method for the establishment and propagation of human brain stem cells from whatever brain tissue samples we have tried. We describe virtually unlimited expansion of an authentic stem cell phenotype. Pluripotency proteins Sox2 and Oct4 are expressed without artificial induction. For the first time multipotency of adult human brain-derived stem cells is demonstrated beyond tissue boundaries. We characterize these cells in detail in vitro including microarray and proteomic approaches. Whilst clarification of these cells’ behavior is ongoing, results so far portend well for the future repair of tissues by transplantation of an adult patient’s own-derived stem cells. |
| Databáze: | OpenAIRE |
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