A simplified system for generating recombinant adenoviruses
| Autor: | Tong-Chuan He, Shibin Zhou, Luis T. da Costa, Jian Yu, Kenneth W. Kinzler, Bert Vogelstein |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Recombinant Fusion Proteins
viruses Genetic Vectors Green Fluorescent Proteins Biology Kidney Transfection medicine.disease_cause Polymerase Chain Reaction Adenoviridae Cell Line law.invention Green fluorescent protein Plasmid Genes Reporter law Escherichia coli medicine Animals Humans Repetitive Sequences Nucleic Acid Mammals Recombination Genetic Multidisciplinary Biological Sciences Virology Cell biology Luminescent Proteins Cell culture Recombinant DNA Homologous recombination Plasmids |
| Zdroj: | Proceedings of the National Academy of Sciences. 95:2509-2514 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.95.5.2509 |
| Popis: | Recombinant adenoviruses provide a versatile system for gene expression studies and therapeutic applications. We report herein a strategy that simplifies the generation and production of such viruses. A recombinant adenoviral plasmid is generated with a minimum of enzymatic manipulations, using homologous recombination in bacteria rather than in eukaryotic cells. After transfections of such plasmids into a mammalian packaging cell line, viral production is conveniently followed with the aid of green fluorescent protein, encoded by a gene incorporated into the viral backbone. Homogeneous viruses can be obtained from this procedure without plaque purification. This system should expedite the process of generating and testing recombinant adenoviruses for a variety of purposes. |
| Databáze: | OpenAIRE |
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