Application of slicing of one-dimensional gels with subsequent slice-by-slice mass spectrometry for the proteomic profiling of human liver cytochromes P450
| Autor: | Alexander I. Archakov, V. G. Zgoda, Andrey V. Lisitsa, Aleksey L. Chernobrovkin, Sergei A. Moshkovskii, Irina I. Karuzina, Herbert Thiele, Natalia A. Petushkova, Oleg G. Skipenko |
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| Rok vydání: | 2009 |
| Předmět: |
Proteomics
Chromatography Human liver Chemistry Proteomic Profiling Molecular Sequence Data General Chemistry Mass spectrometry Biochemistry Slicing Mass Spectrometry Electrophoresis Cytochrome P-450 Enzyme System Liver Microsomes Liver Humans Protein identification Electrophoresis Polyacrylamide Gel Amino Acid Sequence Peptides |
| Zdroj: | Journal of proteome research. 9(1) |
| ISSN: | 1535-3907 |
| Popis: | Sequential thin slicing of one-dimensional electrophoresis gels followed by slice-by-slice mass spectrometry to allow protein identification was used to produce a proteomic map for cytochromes P450. Parallel MALDI-TOF-MS and LC-MS/MS analyses were performed. Combination of the two MS methods increased the quality of protein identification. We have proposed an efficient approach to obtain a comprehensive profile of drug-metabolizing enzymes in the liver that can be used to differentiate between polymorphic variants of cytochromes P450. |
| Databáze: | OpenAIRE |
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