The automated bone marrow micronucleus test
Autor: | F Romagna, C D Staniforth |
---|---|
Rok vydání: | 1989 |
Předmět: |
Pathology
medicine.medical_specialty Health Toxicology and Mutagenesis Population Biology Flow cytometry Mice Bone Marrow Image Processing Computer-Assisted Genetics medicine Animals education Molecular Biology education.field_of_study Micronucleus Tests medicine.diagnostic_test Flow Cytometry Rats Inbred F344 Rats Red blood cell Haematopoiesis medicine.anatomical_structure Immunology Micronucleus test Bone marrow Micronucleus Percoll |
Zdroj: | Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis. 213:91-104 |
ISSN: | 0027-5107 |
Popis: | A new technology is presented which offers high-quality slides enabling the fully automated scoring of large quantities of erythrocytic cells for micronuclei by computerized image analysis. The techniques are applicable to bone marrow specimens as well as to peripheral blood obtained from various species of laboratory animals as well as from man. The key steps leading to this improved slide quality are the total removal of nucleated hematopoietic cells and the production of 'flat' cells by cytocentrifugation on polylysine-coated slides. The new procedures also allow the quantitative elimination of artifact-producing leukocytic granules from the rat bone marrow, even for the Fischer-344 strain, thus making the rat micronucleus test an attractive system for routine purposes in genetic toxicology. In addition, the proportion of immature erythrocytes can, if desired, be increased to more than 90% by using a Percoll step-gradient. This greatly facilitates the peripheral blood micronucleus test in laboratory animals as well as in (splenectomized) humans. First results, using peripheral blood from 2 rat strains, indicate that the immature erythrocyte population is very useful for micronucleus analysis, which encourages the development of a rat peripheral blood micronucleus test. This is an interesting application because it allows repeated testing in the same animals, resulting in fewer rats being needed, as no separate control groups are necessary. A further advantage is the possibility of concomitantly using rats from an ongoing toxicological study for micronucleus testing. The present results demonstrate that the new methodology is a valuable tool for improved micronucleus testing. Possible consequences in the field of genetic toxicology are discussed. |
Databáze: | OpenAIRE |
Externí odkaz: |