Association of IL4R-rs1805016 and IL6R-rs8192284 polymorphisms with clinical dengue in children from Colombian populations
| Autor: | Gabriel Bedoya, Carlos F. Narváez, Doris M. Salgado, Omer Campo, Yerly M. Useche, Berta Nelly Restrepo |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Adult Male Adolescent Genotype 030106 microbiology Population Single-nucleotide polymorphism Ancestry-informative marker Biology Colombia Polymerase Chain Reaction Polymorphism Single Nucleotide Dengue fever lcsh:Infectious and parasitic diseases Dengue 03 medical and health sciences 0302 clinical medicine Gene Frequency Genetic predisposition medicine Humans Genetic Predisposition to Disease lcsh:RC109-216 030212 general & internal medicine education Child Allele frequency Genotyping Genetic Association Studies Genetics education.field_of_study lcsh:Public aspects of medicine Public Health Environmental and Occupational Health Infant Interleukin-4 Receptor alpha Subunit lcsh:RA1-1270 General Medicine medicine.disease Receptors Interleukin-6 Infectious Diseases Case-Control Studies Child Preschool Female Polymorphism Restriction Fragment Length |
| Zdroj: | Journal of Infection and Public Health, Vol 12, Iss 1, Pp 43-48 (2019) |
| ISSN: | 1876-0341 |
| Popis: | Background: Etiologic studies provide evidence that IL-4R and IL-6R receptors may play important roles in the regulatory mechanisms of the development of clinical dengue, especially in children which is a segment of the population with high severe dengue risk. Moreover, the allele frequencies and genetic associations may be influenced by the populational genetic background. Therefore, we performed a case-control study to evaluate possible associations between SNPs in IL4R and IL6R genes and clinical dengue in children from two Colombian populations. Methods: We genotyped the rs1805016 (IL4R) and rs8192284 (IL6R) by PCR-RFLP method, in 298 symptomatic children and 648 asymptomatic controls. Three individual genetic ancestral proportions (APs) (European, Amerindian, African) were inferred by genotyping 29 AIMs (Ancestry informative markers). The variables gender, APs, and the population of origin were used like confusion variables. Results: We found IL4R-rs1805016 GG genotype and G-allele carriers and IL6R-rs8192284 AA genotype associated with clinical dengue in the pooled and Huila samples. Nevertheless, we found no association of these polymorphisms in the sample of Antioquia. Conclusions: For the first time, we report SNPs in IL4R and IL6R genes associated with clinical dengue, which contributes to understanding the genetic susceptibility to dengue disease. Moreover, these results may be influenced by genetic background and must be evaluated through functional analysis. Keywords: SNPs, Dengue, IL4R, IL6R, Th2 response |
| Databáze: | OpenAIRE |
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