Is blood agar an alternative to sabouraud dextrose agar for the isolation of fungi in patients with mycotic keratitis
| Autor: | Milind Bhide, Upputuri Brahmaiah, Syed Maaz Mohiuddin, Ravi Kumar Reddy, Ashok Kumar Reddy, Srinivas Prasad, B S R Murthy, Madhukar K. Reddy, Meghraj Chitta, Murali K Aasuri, Sajid Ahmed, Rishi Swarup, Rupak Kumar Reddy, Nitesh Narayen |
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| Rok vydání: | 2012 |
| Předmět: |
food.ingredient
Fungus Keratitis Microbiology Agar plate Chocolate agar chemistry.chemical_compound food medicine Humans Agar Fungal keratitis Cacao biology Inoculation Fungi medicine.disease biology.organism_classification Isolation (microbiology) Culture Media Ophthalmology Blood Glucose chemistry Eye Infections Fungal |
| Zdroj: | International Ophthalmology. 33:251-254 |
| ISSN: | 1573-2630 0165-5701 |
| DOI: | 10.1007/s10792-012-9683-5 |
| Popis: | To compare the blood agar (BA), sabouraud dextrose agar (SDA) and chocolate agar (CA) for the isolation of fungi in patients with mycotic keratitis. Corneal Scrapings of 229 patients with clinically diagnosed microbial keratitis were inoculated on BA, SDA, CA. The culture media were evaluated for the rate and time taken for the fungal growth. Seventy six of 229 patients had fungal keratitis. Fungus grew on BA in 60/76(78.9 %), on SDA in 76/76 (100 %), on CA in 40/76(52.6 %) patients. The fungi which grew on BA (60/76) also grown on SDA at the same time. The colony morphologies of different fungi were better on SDA than BA/CA. Among the different culture media, SDA is essential for the isolation fungi in patients with mycotic keratitis. |
| Databáze: | OpenAIRE |
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