Purification of class I medullipins from the venous effluent of isolated normal kidneys perfused under high pressure with saline
| Autor: | Krishna R. Maddipati, E E Muirhead, Kirk M. Maxey, Michael Muirhead, B Brooks, L W Byers, James A. Pitcock |
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| Rok vydání: | 1994 |
| Předmět: |
Male
medicine.medical_specialty medicine.medical_treatment Prohormone Sodium Chloride Kidney High-performance liquid chromatography Renal Veins Rats Inbred SHR Internal Medicine medicine Pressure Animals Rats Wistar Saline Effluent Chromatography High Pressure Liquid Medullipin I Chromatography business.industry Albumin General Medicine Lipids Surgery Rats Perfusion High pressure Chromatography Thin Layer Rabbits Cardiology and Cardiovascular Medicine business Normal kidneys medicine.drug |
| Zdroj: | Blood pressure. 3(6) |
| ISSN: | 0803-7051 |
| Popis: | Medullipin I (Med I) is a vasodepressor prohormone which is continuously elaborated into the renal venous effluent (RVE) of isolated rat kidneys perfused under high pressure. We have improved the yield of Med I by substituting saline for the albumin perfusate previously reported; and considerably improved refinement by directly fractionating the crude lipid extract of the RVE with high pressure liquid chromatography. The results show that Med I, as defined by previous physiologic and pharmacologic criteria, is not a single molecule. The 3 Class I medullipins described here are distinguished by subtle or overt differences in polarity and biologic activity. |
| Databáze: | OpenAIRE |
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