Isolation of functional human trophoblast cells and their partial characterization in primary cell culture
| Autor: | Kurt Stromberg, J. C. Azizkhan, Kermit V Speeg |
|---|---|
| Rok vydání: | 1978 |
| Předmět: |
endocrine system
medicine.medical_specialty medicine.drug_class Cell Cell Separation Plant Science Biology Chorionic Gonadotropin Cell Line Andrology chemistry.chemical_compound Theophylline Internal medicine Cyclic AMP medicine Humans Cyclic adenosine monophosphate Centrifugation Secretion Cells Cultured reproductive and urinary physiology Choriocarcinoma Trophoblast medicine.disease Trophoblasts Methotrexate medicine.anatomical_structure Endocrinology chemistry Cell culture embryonic structures Gonadotropin hormones hormone substitutes and hormone antagonists Biotechnology |
| Zdroj: | In Vitro. 14:631-638 |
| ISSN: | 1475-2689 0073-5655 |
| DOI: | 10.1007/bf02617924 |
| Popis: | Human trophoblast isolation and cell culture procedures were examined to identify variables that enhance secretion of chorionic gonadotropin (HCG) in primary culture. Brief exposure of unminced first-trimester placental specimens to a solution of trypsin-EDTA-DNAse, and isolation of the dispersed cells after Ficoll-hypaque centrifugation yielded primary cultures that were high in HCG secretion and content of epithelial-like cells. The gradual decline in HCG level with time in monolayer culture in these presumptive trophoblast cells was retarded by treatment with theophylline and cyclic adenosine monophosphate. Exposure to methotrexate (MTX) did not increase HCG secretion in normal trophoblast cells, in contrast to a 5-fold stimulation by MTX in the JAR line of choriocarcinoma cells. Clusters of polygonal cells in primary culture progressively lost their capacity to secrete HCG and their epithelial-like morphology. However, they could be maintained as cell strains through approximately 15 passages over a period of 13 to 16 weeks. |
| Databáze: | OpenAIRE |
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