Gene induction during differentiation of human monocytes into dendritic cells: an integrated study at the RNA and protein levels

Autor: Catherine Angénieux, Dominique Fricker, Jean-Marc Strub, Sylvie Luche, Huguette Bausinger, Jean-Pierre Cazenave, Alain Van Dorsselaer, Daniel Hanau, Henri de la Salle, Thierry Rabilloud
Přispěvatelé: Biologie des cellules dendritiques humaines, Institut National de la Santé et de la Recherche Médicale (INSERM), Substances naturelles/chimie moléculaire, Université Louis Pasteur - Strasbourg I-Ecole européenne de chimie, polymères et matériaux [Strasbourg]-Centre National de la Recherche Scientifique (CNRS), Bioénergétique Cellulaire et Pathologique (BECP), Université Joseph Fourier - Grenoble 1 (UJF)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)
Jazyk: angličtina
Rok vydání: 2001
Předmět:
MESH: Cell Differentiation
MESH: DNA Primers
Cell signaling
Proteome
Biology
MESH: Monocytes
Article
03 medical and health sciences
transcriptomics
MESH: Gene Expression Profiling
proteomics
MESH: RNA
MESH: Reverse Transcriptase Polymerase Chain Reaction
[SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Genomics [q-bio.GN]
Gene expression
Genetics
Protein biosynthesis
Humans
Quantitative Biology - Genomics
MESH: Proteins
Gene
DNA Primers
030304 developmental biology
Genomics (q-bio.GN)
0303 health sciences
MESH: Humans
denditic cells
MESH: Dendritic Cells
Reverse Transcriptase Polymerase Chain Reaction
Antigen processing
Gene Expression Profiling
030302 biochemistry & molecular biology
Proteins
RNA
Cell Differentiation
MESH: Comparative Study
Dendritic Cells
General Medicine
differentiation
superoxide dismutase
Cell biology
MESH: Proteome
FOS: Biological sciences
Signal transduction
monocytes
Function (biology)
Zdroj: Funct Integr Genomics
Funct Integr Genomics, 2001, 1 (5), pp.323-9. ⟨10.1007/s101420100037⟩
DOI: 10.1007/s101420100037⟩
Popis: Changes in gene expression occurring during differentiation of human monocytes into dendritic cells were studied at the RNA and protein levels. These studies showed the induction of several gene classes corresponding to various biological functions. These functions encompass antigen processing and presentation, cytoskeleton, cell signalling and signal transduction, but also an increase in mitochondrial function and in the protein synthesis machinery, including some, but not all, chaperones. These changes put in perspective the events occurring during this differentiation process. On a more technical point, it appears that the studies carried out at the RNA and protein levels are highly complementary.
website publisher: http://www.springerlink.com/content/ha0d2c351qhjhjdm/
Databáze: OpenAIRE
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