Different metabolite profile and metabolic pathway with leaves and roots in response to boron deficiency at the initial stage of citrus rootstock growth
| Autor: | Lishu Wu, Riaz Muhammad, Xiaopei Lu, Asad Hussain Shah, Xiuwen Wu, Xiaochang Dong, Cuncang Jiang, Guidong Liu, Lei Yan |
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| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine Physiology Shikimic Acid Plant Science Pentose phosphate pathway Plant Roots 01 natural sciences Gas Chromatography-Mass Spectrometry Pentose Phosphate Pathway 03 medical and health sciences Genetics Metabolomics Poncirus Shikimate pathway Amino Acids Citrus rootstock Amino acid synthesis Boron chemistry.chemical_classification biology Plant physiology Metabolism biology.organism_classification Trifoliate orange Plant Leaves Metabolic pathway 030104 developmental biology chemistry Biochemistry Seedlings Metabolic Networks and Pathways 010606 plant biology & botany |
| Zdroj: | Plant Physiology and Biochemistry. 108:121-131 |
| ISSN: | 0981-9428 |
| Popis: | Boron (B) is a microelement required for higher plants, and B deficiency has serious negative effect on metabolic processes. We concentrated on the changes in metabolite profiles of trifoliate orange leaves and roots as a consequence of B deficiency at the initial stage of growth by gas chromatography-mass spectrometry (GC-MS)-based metabolomics. Enlargement and browning of root tips were observed in B-deficient plants, while any obvious symptom was not recorded in the leaves after 30 days of B deprivation. The distinct patterns of alterations in metabolites observed in leaves and roots due to B deficiency suggest the presence of specific organ responses to B starvation. The accumulation of soluble sugars was occurred in leaves, which may be attributed to down-regulated pentose phosphate pathway (PPP) and amino acid biosynthesis under B deficiency, while the amount of most amino acids in roots was increased, indicating that the effects of B deficiency on amino acids metabolism in trifoliate orange may be a consequence of disruptions in root tissues and decreased protein biosynthesis. Several important products of shikimate pathway were also significantly affected by B deficiency, which may be related to abnormal growth of roots induced by B deficiency. Conclusively, our results revealed a global perspective of the discriminative metabolism responses appearing between B-deprived leaves and roots and provided new insight into the relationship between B deficiency symptom in roots and the altered amino acids profiling and shikimate pathway induced by B deficiency during seedling establishment. |
| Databáze: | OpenAIRE |
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