A novel functional rabbit IL-7 isoform
| Autor: | Susan L. Kalis, Tong Zhou, Basile T. Siewe, Katherine L. Knight, Pedro J. Esteves |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Cellular differentiation
T cell T-Lymphocytes Immunology Bone Marrow Cells Biology Lymphocyte Activation Article TCIRG1 Exon Mice Cell Line Tumor medicine STAT5 Transcription Factor Animals Humans Protein Isoforms Lymphopoiesis Progenitor cell B cell Janus Kinases B-Lymphocytes Hybridomas Receptors Interleukin-7 Interleukin-7 Alternative splicing Molecular biology Recombinant Proteins Alternative Splicing medicine.anatomical_structure Rabbits Developmental Biology Protein Binding Signal Transduction |
| Zdroj: | Developmental and comparative immunology. 34(8) |
| ISSN: | 1879-0089 |
| Popis: | IL-7 is required for B cell development in mouse and is a key regulator of T cell development and peripheral T cell homeostasis in mouse and human. Recently, we found that IL-7 is expressed in rabbit bone marrow and in vitro, is required for differentiation of lymphoid progenitors to B and T lineage cells. Herein, we report the identification of a novel rabbit IL-7 isoform, IL-7II. Recombinant IL-7II (rIL-7II) binds lymphocytes via the IL-7R and induces phosphorylation of STAT5. Further, rIL-7II supports proliferation and differentiation of BM progenitor cells into B and T lineage cells. IL7-II is generated by alternative splicing, with an 11 amino acid insertion encoded by a separate exon, exon 2b. Exon 2b is conserved in other lagomorphs, in Perissodactyla, Artiodactyla, and Carnivora, but is absent in mouse and human. |
| Databáze: | OpenAIRE |
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