Structure and regulation of gene expression of a Clo DF13 plasmid DNA region involved in plasmid segregation and incompatibility
| Autor: | Hakkaart Mj, Peter J.M. van den Elzen, E. Veltkamp, Hanneke H.B. Walters, H. John J. Nijkamp, Arnold J. van Putten |
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| Rok vydání: | 1983 |
| Předmět: |
Genetics
Regulation of gene expression Transposable element Base Sequence Transcription Genetic Operon Single-Strand Specific DNA and RNA Endonucleases Nucleic Acid Hybridization DNA Restriction Enzymes Biology Endonucleases Molecular biology Plasmid Bacteriocins Genes Transcription (biology) Genes Bacterial Gene expression DNA Transposable Elements Escherichia coli Repressor lexA Amino Acid Sequence Gene Plasmids |
| Zdroj: | Nucleic acids research. 11(24) |
| ISSN: | 0305-1048 |
| Popis: | The bacteriocinogenic plasmid Clo DF13 contains genetic information involved in the accurate partitioning of the plasmid (parA and parB) as well as in incompatibility phenomena (incA, B, C and D). In this paper we report on the primary structure and regulation of gene expression of the 29% - 50% part of Clo DF13, containing the DNA regions incA, incB and parB as well as genes K and L. According to the results of our DNA sequence analysis, mapping of transposon insertions, RNA blotting and S1 mapping experiments, we conclude that: a) genes K and L are transcribed as one operon; transcription of this operon is initiated at a promoter (P2) located at 32.5% and proceeds in a clockwise direction. b) treatment of cells with mitomycin-C, significantly enhances transcription from P2, although this promoter is probably not directly repressed by lexA protein. c) Termination of transcription of this operon occurs between genes K and L, as well as distal to gene L. The possible role of gene products and/or sites, located within the 29-50% DNA region, in plasmid incompatibility and segregation is discussed. |
| Databáze: | OpenAIRE |
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