Apolipophorin-III-like protein expressed in the antenna of the red imported fire ant,Solenopsis invicta Buren (Hymenoptera: Formicidae)
| Autor: | J. Aaron Cassill, Daniel Velasquez, Linda V. Chadwell, Christopher A. Carroll, Susan E Weintraub, Robert Renthal, Kalyani V. P. Guntur |
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| Rok vydání: | 2004 |
| Předmět: |
Male
Fire ant Physiology Sequence analysis Molecular Sequence Data Sequence alignment Biochemistry Mass Spectrometry Red imported fire ant Organophosphorus Compounds Protein sequencing Complementary DNA Botany Animals Electrophoresis Gel Two-Dimensional Amino Acid Sequence Peptide sequence DNA Primers Base Sequence biology Edman degradation Ants Reverse Transcriptase Polymerase Chain Reaction fungi Sense Organs Sequence Analysis DNA General Medicine biology.organism_classification Molecular biology Apolipoproteins Insect Science Sequence Alignment |
| Zdroj: | Archives of Insect Biochemistry and Physiology. 57:101-110 |
| ISSN: | 1520-6327 0739-4462 |
| DOI: | 10.1002/arch.20019 |
| Popis: | Antennal proteins of the male fire ant (Solenopsis invicta) were analyzed by two-dimensional gel electrophoresis, with the objective of identifying pheromone-binding proteins, which have not previously been found in ant antennae. The major low-molecular weight protein found in the male fire ant antenna was subjected to Edman degradation to determine the N-terminal amino acid sequence. Degenerate PCR primers based on this sequence were used to obtain a cDNA sequence corresponding to the full-length protein sequence. In-gel trypsin digestion followed by MALDI-TOF mass spectrometry and HPLC-ESI/MS/MS demonstrated that the protein gel spot contained only the protein corresponding to the cDNA sequence obtained by PCR. The sequence is similar to apolipophorin-III, an exchangeable lipid-binding protein. Fire ant apolipophorin-III is expressed in the antenna as well as the head, thorax and abdomen. |
| Databáze: | OpenAIRE |
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