Detection of classical and atypical/Nor98 scrapie by the paraffin-embedded tissue blot method
| Autor: | Wilhelm Wemheuer, Wiebke M. Wemheuer, Bertram Brenig, Sylvie L. Benestad, Bjørn Bratberg, Walter J. Schulz-Schaeffer, Arne Wrede |
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| Rok vydání: | 2009 |
| Předmět: |
Central Nervous System
Pathology medicine.medical_specialty Lymphoid Tissue Prions 040301 veterinary sciences Blotting Western Palatine Tonsil Central nervous system Scrapie Biology Sensitivity and Specificity Palatine tonsil 0403 veterinary science 03 medical and health sciences medicine Animals 030304 developmental biology 0303 health sciences Paraffin Embedding Sheep General Veterinary Brain 04 agricultural and veterinary sciences General Medicine Classical scrapie Immunohistochemistry Paraffin embedded tissue Blot medicine.anatomical_structure Lymphatic system Case-Control Studies |
| Zdroj: | Veterinary Record. 164:677-681 |
| ISSN: | 0042-4900 |
| DOI: | 10.1136/vr.164.22.677 |
| Popis: | The paraffin-embedded tissue (PET) blot method was used to investigate sections of the central nervous system and lymphatic tissues from 24 cases of classical scrapie and 25 cases of atypical/Nor98 scrapie in sheep and four healthy control sheep. The PET blot detected deposits of PrP(Sc) in the brain tissue of all 49 sheep with scrapie but no PrP(Sc) labelling could be detected in the control sheep. By contrast, not all the atypical/Nor98 scrapie cases were detectable by immunohistochemistry. The high sensitivity of the PET blot method made it possible to observe that in some atypical/Nor98 cases, deposits of PrP(Sc) may be restricted to supratentorial brain structures and that the diagnosis may be missed when only testing the obex area, where deposits are common in classical scrapie, and the cerebellar structures, where deposits are considered to be common in atypical/Nor98 cases. |
| Databáze: | OpenAIRE |
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