Prevalence of murine astrovirus in laboratory animal facilities in Japan
| Autor: | Nobuhito Hayashimoto, Hanako Morita, Masahiko Yasuda, Mai Tanaka, Ritsuki Uchida, Tomoko Ishida, Yamamoto Masafumi |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
040301 veterinary sciences
prevalence Biology Disease cluster Polymerase Chain Reaction law.invention 0403 veterinary science Rodent Diseases 03 medical and health sciences Cecum chemistry.chemical_compound Immunocompromised Host Mice Japan law Laboratory Animal Science RNA polymerase Animals Laboratory Astroviridae Infections medicine Animals Gene Polymerase chain reaction Phylogeny 030304 developmental biology 0303 health sciences laboratory mice General Veterinary Phylogenetic tree 04 agricultural and veterinary sciences Note Virology murine astrovirus medicine.anatomical_structure chemistry Astroviridae Murine astrovirus |
| Zdroj: | The Journal of Veterinary Medical Science |
| ISSN: | 1347-7439 0916-7250 |
| Popis: | To investigate the prevalence of murine astrovirus (MuAstV) in mice in laboratory animal facilities in Japan, a polymerase chain reaction (PCR) test targeting the RNA-dependent RNA polymerase (RdRP) gene was performed on the cecum contents of 1,212 mice (1,183 immunocompetent mice and 29 immunodeficient mice) from 226 facilities. The results showed that 118 (52.2%) of the 226 facilities were positive for MuAstV. Out of the 1,212 mice, 424 (35.0%) were positive. No gross lesions were observed in any of the mice examined. A phylogenetic analysis for 15 selected strains revealed that 13 strains formed one cluster, while two were genetically distant from that cluster. These results suggest that multiple strains are prevalent in laboratory mice in Japan. |
| Databáze: | OpenAIRE |
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