The endocannabinoid 2-AG controls skeletal muscle cell differentiation via CB1 receptor-dependent inhibition of Kv7 channels
| Autor: | Tamás Bíró, Daniela Calvigioni, Vincenzo Di Marzo, Gabriella Czifra, Paolo Ambrosino, Fabio Arturo Iannotti, Fabiana Piscitelli, Enrico Mazzarella, Tibor Harkany, Andrea Martella, Cristoforo Silvestri, Maurizio Taglialatela, Stefania Petrosino |
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| Přispěvatelé: | Iannotti, Fa, Silvestri, C, Mazzarella, E, Martella, A, Calvigioni, D, Piscitelli, F, Ambrosino, P, Petrosino, S, Czifra, G, Bíró, T, Harkany, T, Taglialatela, Maurizio, Di Marzo, V. |
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
medicine.medical_specialty
Cannabinoid receptor Inositol Phosphates Myoblasts Skeletal Cellular differentiation Muscle Fibers Skeletal Arachidonic Acids CHO Cells Biology Muscle Development Transfection Glycerides Receptor Cannabinoid CB2 Mice Cricetulus Skeletal muscle cell differentiation Receptor Cannabinoid CB1 Cricetinae Internal medicine medicine Animals Humans Myocyte Gene Silencing Elméleti orvostudományok Muscle Skeletal Cell Proliferation Multidisciplinary KCNQ Potassium Channels Myogenesis musculoskeletal neural and ocular physiology Cell Differentiation Orvostudományok Endocannabinoid system Endocrinology PNAS Plus nervous system Silicone Elastomers lipids (amino acids peptides and proteins) Signal transduction C2C12 psychological phenomena and processes Endocannabinoids Signal Transduction |
| Zdroj: | Proceedings of the National Academy of Sciences of the United States of America (2014). doi:10.1021/cn5000524 info:cnr-pdr/source/autori:Fabio A. Iannotti, Cristoforo Silvestri, Enrico Mazzarella, Andrea Martella, Daniela Calvigioni, Fabiana Piscitelli, Paolo Ambrosino, Stefania Petrosino, Gabriella Czifra, Tamás Bíró, Tibor Harkany, Maurizio Taglialatela, and Vincenzo Di Marzo/titolo:The endocannabinoid 2-AG controls skeletal muscle cell differentiation via CB1 receptor-dependent inhibition of Kv7 channels./doi:10.1021%2Fcn5000524/rivista:Proceedings of the National Academy of Sciences of the United States of America/anno:2014/pagina_da:/pagina_a:/intervallo_pagine:/volume |
| DOI: | 10.1021/cn5000524 |
| Popis: | Little is known of the involvement of endocannabinoids and cannabinoid receptors in skeletal muscle cell differentiation. We report that, due to changes in the expression of genes involved in its metabolism, the levels of the endocannabinoid 2-arachidonoylglycerol (2-AG) are decreased both during myotube formation in vitro from murine C2C12 myoblasts and during mouse muscle growth in vivo. The endocannabinoid, as well as the CB1 agonist arachidonoyl-2-chloroethylamide, prevent myotube formation in a manner antagonized by CB1 knockdown and by CB1 antagonists, which, per se, instead stimulate differentiation. Importantly, 2-AG also inhibits differentiation of primary human satellite cells. Muscle fascicles from CB1 knockout embryos contain more muscle fibers, and postnatal mice show muscle fibers of an increased diameter relative to wild-type littermates. Inhibition of Kv7.4 channel activity, which plays a permissive role in myogenesis and depends on phosphatidylinositol 4,5-bisphosphate (PIP2), underlies the effects of 2-AG. We find that CB1 stimulation reduces both total and Kv7.4-bound PIP2 levels in C2C12 cells and inhibits Kv7.4 currents in transfected CHO cells. We suggest that 2-AG is an endogenous repressor of myoblast differentiation via CB1-mediated inhibition of Kv7.4 channels. |
| Databáze: | OpenAIRE |
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