Anti-Autophagy Mechanism of Zhi Gan Prescription Based on Network Pharmacology in Nonalcoholic Steatohepatitis Rats
| Autor: | Zhuofan Weng, Zhaojun Wang, Lichuan Luo, Yusheng Cui, Beilei Li, Chufeng Qin, Cuiting Weng, Yijie Lou, Chenxin Liu, Jingwen Lou, Yunxi Ji, Li Jiang |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Nonalcoholic steatohepatitis autophagy zhi gan prescription molecular mechanisms RM1-950 Pharmacology digestive system Parkin 03 medical and health sciences 0302 clinical medicine Western blot Network pharmacology medicine PTEN network pharmacology Pharmacology (medical) Original Research biology medicine.diagnostic_test Mechanism (biology) Chemistry Autophagy nutritional and metabolic diseases digestive system diseases 030104 developmental biology mitochondrial fusion biology.protein 030211 gastroenterology & hepatology Therapeutics. Pharmacology non-alcoholic steatohepatitis |
| Zdroj: | Frontiers in Pharmacology Frontiers in Pharmacology, Vol 12 (2021) |
| ISSN: | 1663-9812 |
| Popis: | Background and Aims: Zhi Gan prescription (ZGP) has been clinically proven to exert a favorable therapeutic effect on nonalcoholic steatohepatitis (NASH). This study purpose to reveal the underlying molecular mechanisms of ZGP action in NASH.Methods: Systematic network pharmacology was used to identify bioactive components, potential targets, and the underlying mechanism of ZGP action in NASH. High fat (HF)-induced NASH model rats were used to assess the effect of ZGP against NASH, and to verify the possible molecular mechanisms as predicted by network pharmacology.Results: A total of 138 active components and 366 potential targets were acquired in ZGP. In addition, 823 targets of NASH were also screened. In vivo experiments showed that ZGP significantly improved the symptoms in HF-induced NASH rats. qRT-PCR and western blot analyses showed that ZGP could regulate the hub genes, PTEN, IL-6 and TNF in NASH model rats. In addition, ZGP suppressed mitochondrial autophagy through mitochondrial fusion and fission via the PINK/Parkin pathway.Conclusion: ZGP exerts its effects on NASH through mitochondrial autophagy. These findings provide novel insights into the mechanisms of ZGP in NASH. |
| Databáze: | OpenAIRE |
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