Knockdown of ZNF479 inhibits proliferation and glycolysis of gastric cancer cells through regulating β‐catenin/ c‐Myc signaling pathway
| Autor: | Rongzhou Li, Zheng-Chao Shi, Wei-Lai Yu, Qing-Qing Zhang, Tingting Ji, Fang-Peng Ye, Xiao-Sheng Jin |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Medicine (General)
proliferation Cell Proto-Oncogene Proteins c-myc 03 medical and health sciences R5-920 0302 clinical medicine Stomach Neoplasms Cell Line Tumor Humans Medicine ZNF479 beta Catenin Cell Proliferation Gene knockdown Oncogene Cell growth business.industry gastric cancer Cancer General Medicine medicine.disease DNA-Binding Proteins medicine.anatomical_structure c‐Myc Gene Knockdown Techniques 030220 oncology & carcinogenesis Catenin Cancer cell Cancer research 030211 gastroenterology & hepatology Signal transduction business Glycolysis Signal Transduction Transcription Factors |
| Zdroj: | Kaohsiung Journal of Medical Sciences, Vol 37, Iss 9, Pp 759-767 (2021) |
| ISSN: | 2410-8650 1607-551X |
| DOI: | 10.1002/kjm2.12399 |
| Popis: | Gastric cancer is the fifth most common malignancy and the third most deadly tumor in the world. Zinc finger protein 479 (ZNF479) has been demonstrated to play crucial roles in hepatocellular carcinoma. However, the function of ZNF479 in gastric cancer remains to be clarified. The current study aimed to investigate the role of ZNF479 in gastric cancer progression and elucidate the potential molecular mechanism. In this study, Cell Count Kit‐8 and colony formation assays demonstrated that knockdown of ZNF479 inhibited cell proliferation in AGS and SGC‐7901 cells. Of note, knockdown of ZNF479 hinders tumor growth of xenograft tumor mice. What is more, knockdown of ZNF479 inhibited glucose uptake, lactate production, adenosine triphosphate level, and extracellular acidification ratio; increased oxygen consumption ratio in gastric cancer cells; and decreased the expression of glycolytic proteins both in vitro and in vivo. Furthermore, analysis mechanism suggests that ZNF479 participated in the regulation of gastric cancer progression through affecting the β‐catenin/c‐Myc signaling pathway. Collectively, ZNF479 plays a role as an oncogene through modulating β‐catenin/c‐Myc signaling pathway in the development of gastric cancer, which provides a new research target for future studies. |
| Databáze: | OpenAIRE |
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