Effect of three diaryl tellurides, and an organoselenium compound in trout erythrocytes exposed to oxidative stress in vitro
| Autor: | Luca Tiano, Donatella Fedeli, Milena Villarini, Lars Engman, Anna Maria Santroni, Giancarlo Falcioni |
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| Jazyk: | angličtina |
| Rok vydání: | 2000 |
| Předmět: |
Azoles
Diaryl tellurides Erythrocytes Hot Temperature Antioxidant DNA damage Health Toxicology and Mutagenesis medicine.medical_treatment Trout erythrocytes Isoindoles medicine.disease_cause Hemolysis Antioxidants Oxidative stress Comet assay DNA Lipid peroxidation chemistry.chemical_compound Organoselenium Compounds Organometallic Compounds Genetics medicine Animals chemistry.chemical_classification Dose-Response Relationship Drug biology Mutagenicity Tests Ebselen Glutathione peroxidase Hydrogen-Ion Concentration biology.organism_classification Trout chemistry Biochemistry Oncorhynchus mykiss Luminescent Measurements Tellurium DNA Damage |
| Popis: | Previous literature reports have demonstrated that nucleated trout erythrocytes in conditions of oxidative stress are subjected to DNA and membrane damage, and inactivation of glutathione peroxidase. The present study was undertaken to evaluate the ability of three diaryl tellurides and the organoselenium compound ebselen to protect trout (Salmo irideus) erythrocytes against oxidative stress, induced thermally and by a variation of pH. The antioxidant ability of these molecules was evaluated through chemiluminescence. Impairment of DNA was assessed using the comet assay, a rapid and sensitive single cell gel electrophoresis technique, used to detect primary DNA damage in individual cells. At low concentrations (10 microM), all the compounds used presented a protective effect on DNA damage without altering the hemolysis rate. In higher concentrations, they accelerated the hemolysis rate and two of the diaryl tellurides were strongly genotoxic. |
| Databáze: | OpenAIRE |
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