CD300lf is the primary physiologic receptor of murine norovirus but not human norovirus
| Autor: | Megan T. Baldridge, Madison S. Simões, Craig B. Wilen, Christiane E. Wobus, Leon L. Hsieh, Abimbola O. Kolawole, Jin Wei, Lisa C. Lindesmith, Elizabeth A. Kennedy, Khalil Ettayebi, Ralph S. Baric, Vincent R. Graziano, Renata B. Filler, Arthur S. Kim, Forrest C. Walker, Robert C. Orchard, Mary K. Estes, Ebrahim Hassan |
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| Rok vydání: | 2020 |
| Předmět: |
RNA viruses
Physiology ved/biology.organism_classification_rank.species Pathology and Laboratory Medicine medicine.disease_cause Mice Immune Physiology Medicine and Health Sciences Biology (General) Receptors Immunologic Receptor Caliciviridae Infections Mice Knockout Viral Genomics 0303 health sciences Mammalian Genomics 030302 biochemistry & molecular biology Genomics 3. Good health Medical Microbiology Viral Pathogens Host-Pathogen Interactions Viruses Viral Genome Receptors Virus Pathogens Anatomy Receptor Physiology Research Article Cell Physiology QH301-705.5 Colon Immunology Microbial Genomics Biology Microbiology Host Specificity Caliciviruses Virus 03 medical and health sciences Extraction techniques Ileum In vivo Virology Genetics medicine Animals Humans Microbial Pathogens Molecular Biology Tropism 030304 developmental biology Biology and life sciences ved/biology Norovirus Organisms Cell Biology RC581-607 RNA extraction Mice Inbred C57BL Gastrointestinal Tract Research and analysis methods Viral Tropism Animal Genomics Viral Receptor Tissue tropism Parasitology Immunologic diseases. Allergy Digestive System Spleen HeLa Cells Murine norovirus |
| Zdroj: | PLoS Pathogens PLoS Pathogens, Vol 16, Iss 4, p e1008242 (2020) |
| ISSN: | 1553-7374 |
| DOI: | 10.1371/journal.ppat.1008242 |
| Popis: | Murine norovirus (MNoV) is an important model of human norovirus (HNoV) and mucosal virus infection more broadly. Viral receptor utilization is a major determinant of cell tropism, host range, and pathogenesis. The bona fide receptor for HNoV is unknown. Recently, we identified CD300lf as a proteinaceous receptor for MNoV. Interestingly, its paralogue CD300ld was also sufficient for MNoV infection in vitro. Here we explored whether CD300lf is the sole physiologic receptor in vivo and whether HNoV can use a CD300 ortholog as an entry receptor. We report that both CD300ld and CD300lf are sufficient for infection by diverse MNoV strains in vitro. We further demonstrate that CD300lf is essential for both oral and parenteral MNoV infection and to elicit anti-MNoV humoral responses in vivo. In mice deficient in STAT1 signaling, CD300lf is required for MNoV-induced lethality. Finally, we demonstrate that human CD300lf (huCD300lf) is not essential for HNoV infection, nor does huCD300lf inhibit binding of HNoV virus-like particles to glycans. Thus, we report huCD300lf is not a receptor for HNoV. Author summary Human norovirus is the leading cause of non-bacterial gastroenteritis causing up to 200,000 deaths each year. How human norovirus enters cells is unknown. Because human norovirus is difficult to grow in the laboratory and in small animals, we use mouse or murine norovirus as a model system. We recently discovered that murine norovirus can use the either CD300ld or CD300lf as a receptor in vitro. We also showed that CD300lf deficient mice were resistant to oral challenge with a single virus strain. Here we determined that CD300lf is essential for infection of diverse murine norovirus strains in cell lines and in mice with normal immune systems demonstrating it’s the primary physiologic receptor for diverse murine norovirus strains independent of infection route. Finally, we demonstrated that human CD300lf is not the elusive proteinaceous receptor for human norovirus. |
| Databáze: | OpenAIRE |
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