Nearest neighbour base frequency of the RNA formed by rat liver DNA-dependent RNA polymerase A and B with homologous DNA
| Autor: | J.R. Tata, E.A. Smuckler |
|---|---|
| Rok vydání: | 1972 |
| Předmět: |
Male
Biophysics RNA-dependent RNA polymerase Biology Cytosine Nucleotides Biochemistry chemistry.chemical_compound Cytosine nucleotide Adenosine Triphosphate Transcription (biology) RNA polymerase Centrifugation Density Gradient Animals Ribonuclease Molecular Biology chemistry.chemical_classification Cell Nucleus Carbon Isotopes Deoxyribonucleases Base Sequence RNA Phosphorus Isotopes Cell Biology DNA DNA-Directed RNA Polymerases Templates Genetic Chromatography Ion Exchange Molecular biology Guanine Nucleotides Rats Enzyme chemistry Liver biology.protein Chromatography Gel Cattle |
| Zdroj: | Biochemical and biophysical research communications. 49(1) |
| ISSN: | 0006-291X |
| Popis: | Four distinct peaks of DNA-dependent RNA polymerase activity were isolated from rat liver nuclei by DEAE-Sephadex chromatography. The product formed by the enzyme in the two major peaks (designated A and B) was subjected to nearest neighbour base frequency analysis. Using identical template (native, homologous DNA) and incubation conditions, it was shown that the product had different nearest neighbour frequencies, using α-32P labelled ATP or α-32P labelled CTP. The RNA product of enzyme A had sequences relatively richer in GMP and CMP than that formed by enzyme B. There was no evidence of ribonuclease activity in the isolated enzymic peaks, and products formed under identical conditions have similar sedimentation characteristics. These results suggest that the enzymic materials may have a recognition mechanism selecting areas for transcription. |
| Databáze: | OpenAIRE |
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