Sex differences in subcellular distribution of delta opioid receptors in the rat hippocampus in response to acute and chronic stress
| Autor: | Elizabeth M. Waters, Teresa A. Milner, Baila S. Hall, Shannon C. Odell, Khalifa Stafford, Jane Selegean, Tracey A. Van Kempen, Sanoara Mazid, Andreina D. Dyer, Bruce S. McEwen |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Dendritic spine CA3 pyramidal cells Interneuron Physiology Hippocampus Biology Hippocampal formation Biochemistry lcsh:RC346-429 lcsh:RC321-571 03 medical and health sciences Cellular and Molecular Neuroscience 0302 clinical medicine Endocrinology Internal medicine Electron microscopy medicine Drug addiction Chronic stress Original Research Article Neural plasticity 10. No inequality lcsh:Neurosciences. Biological psychiatry. Neuropsychiatry Molecular Biology lcsh:Neurology. Diseases of the nervous system Endocrine and Autonomic Systems lcsh:QP351-495 Associative learning GABAergic interneurons lcsh:Neurophysiology and neuropsychology 030104 developmental biology medicine.anatomical_structure GABAergic Pyramidal cell Neuroscience Oxycodone 030217 neurology & neurosurgery |
| Zdroj: | Neurobiology of Stress, Vol 5, Iss C, Pp 37-53 (2016) Neurobiology of Stress |
| ISSN: | 2352-2895 |
| Popis: | Drug addiction requires associative learning processes that critically involve hippocampal circuits, including the opioid system. We recently found that acute and chronic stress, important regulators of addictive processes, affect hippocampal opioid levels and mu opioid receptor trafficking in a sexually dimorphic manner. Here, we examined whether acute and chronic stress similarly alters the levels and trafficking of hippocampal delta opioid receptors (DORs). Immediately after acute immobilization stress (AIS) or one-day after chronic immobilization stress (CIS), the brains of adult female and male rats were perfusion-fixed with aldehydes. The CA3b region and the dentate hilus of the dorsal hippocampus were quantitatively analyzed by light microscopy using DOR immunoperoxidase or dual label electron microscopy for DOR using silver intensified immunogold particles (SIG) and GABA using immunoperoxidase. At baseline, females compared to males had more DORs near the plasmalemma of pyramidal cell dendrites and about 3 times more DOR-labeled CA3 dendritic spines contacted by mossy fibers. In AIS females, near-plasmalemmal DOR-SIGs decreased in GABAergic hilar dendrites. However, in AIS males, near-plasmalemmal DOR-SIGs increased in CA3 pyramidal cell and hilar GABAergic dendrites and the percentage of CA3 dendritic spines contacted by mossy fibers increased to about half that seen in unstressed females. Conversely, after CIS, near-plasmalemmal DOR-SIGs increased in hilar GABA-labeled dendrites of females whereas in males plasmalemmal DOR-SIGs decreased in CA3 pyramidal cell dendrites and near-plasmalemmal DOR-SIGs decreased hilar GABA-labeled dendrites. As CIS in females, but not males, redistributed DOR-SIGs near the plasmalemmal of hilar GABAergic dendrites, a subsequent experiment examined the acute affect of oxycodone on the redistribution of DOR-SIGs in a separate cohort of CIS females. Plasmalemmal DOR-SIGs were significantly elevated on hilar interneuron dendrites one-hour after oxycodone (3 mg/kg, I.P.) administration compared to saline administration in CIS females. These data indicate that DORs redistribute within CA3 pyramidal cells and dentate hilar GABAergic interneurons in a sexually dimorphic manner that would promote activation and drug related learning in males after AIS and in females after CIS. Graphical abstract Image 1 Highlights • Females have more near-plasmalemmal DORs in pyramidal CA3 dendrites than males. • Acute stress in males relocates DORs in CA3 & GABA dendrites to promote activation. • Chronic stress in females relocates DORs in GABA dendrites in females to promote activation. • Chronic stress in males relocates DORs in GABA dendrites opposite of females. • DOR-stress relocation may contribute to sexually dimorphic effects on drug related learning. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|