Multiplicação in vitro de Neoregelia johannis (Carriére) L.B. Smith em meio líquido sob diferentes concentrações de nutrientes

Autor:	Marcílio de Almeida, Eveline Calderan Meneghetti, Leandro Silva de Oliveira
Rok vydání:	2019
Předmět:	Plant growth Neoregelia johannis biology Germplasm Bank food and beverages Bromeliaceae Plant Science REGULADORES DE CRESCIMENTO VEGETAL biology.organism_classification Horticulture Murashige and Skoog medium Nutrient Micropropagation Shoot
Zdroj:	Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP
ISSN:	2446-8231 0073-4705
Popis:	The importance of micropropagation to Bromeliaceae species is increasing, however few studies report the role of nutrition in in vitro multiplication. In order to correlate micropropagation morphophysiological aspects with regards to availability of nutrients, mainly nitrogen, Neoregelia johannis shoots were subcultured in the media culture MS, ½ MS and WPM, supplemented with the same concentrations of plant growth regulators. The multiplication rate was similar for all the culture media, with the highest multiplication rate, in addition to the highest accumulation of total and protein nitrogen, in the MS culture medium. Even producing the smallest shoots, the means of cultivation ½ MS and WPM represent a viable alternative for the maintenance of germplasm bank and for the maintenance of important genotypes in plant biofactories, considering that they present uniform results. The MS medium culture would be recommended for specific cases, when it is necessary to accelerate the multiplication and especially the growth of the shoots.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c6d1a9874a58752c9373e13f4e271c83 https://doi.org/10.21826/2446-82312019v74e2019012 Zobrazit plný text záznamu