Refinement of a model of repeated cerebrospinal fluid collection in conscious rats
| Autor: | Muriel Brecheisen, Eva Maria Amen, Alessandra Bergadano, Lisa Sach-Peltason |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine Central nervous system Animal Welfare Cerebrospinal fluid collection Cisterna magna Catheterization Specimen Handling 03 medical and health sciences Animal.strain 0302 clinical medicine Cerebrospinal fluid Animal welfare Animals Medicine Anesthesia Rats Wistar Cerebrospinal Fluid Repeated sampling General Veterinary business.industry Rats 030104 developmental biology medicine.anatomical_structure Animal Science and Zoology Implant Analgesia business 030217 neurology & neurosurgery |
| Zdroj: | Laboratory Animals. 51:44-53 |
| ISSN: | 1758-1117 0023-6772 |
| DOI: | 10.1177/0023677216646069 |
| Popis: | The cannulation of the cisterna magna in rats for in vivo sampling of cerebrospinal fluid serves as a valuable model for studying the delivery of new drugs into the central nervous system or disease models. It offers the advantages of repeated sampling without anesthesia-induced bias and using animals as their own controls. An established model was retrospectively reviewed for the outcomes and it was hypothesized that by refining the method, i.e. by (1) implementing pathophysiological-based anesthesia and analgesia, (2) using state-of-the-art peri-operative monitoring and supportive care, (3) increasing stability of the cement–cannula assembly, and (4) selecting a more adaptable animal strain, the outcome in using the model – quantified by peri-operative mortality, survival time and stability of the implant – could be improved and could enhance animal welfare. After refinement of the technique, peri-operative mortality decreased significantly (7 animals out of 73 compared with 4 out of 322; P = 0.001), survival time increased significantly (36 ± 14 days compared with 28 ± 18 days; P |
| Databáze: | OpenAIRE |
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