Mechanism of the enrichment of phosphatidylcholine in liver accompanying enzyme induction by phenobarbital
| Autor: | H. Kewitz, Pleul O, Schwartzkopff Th, Schwartzkopff G |
|---|---|
| Rok vydání: | 1977 |
| Předmět: |
Male
Time Factors Pharmacology toxicology Choline Phosphates chemistry.chemical_compound Methionine Phosphatidylcholine medicine Animals Enzyme inducer Pharmacology biology Mechanism (biology) Phosphatidylethanolamines Proteins General Medicine Methylation Lipid Metabolism Rats Liver chemistry Biochemistry Ethanolamines Enzyme Induction Phenobarbital Phosphatidylcholines biology.protein Phosphorus Radioisotopes medicine.drug |
| Zdroj: | Naunyn-Schmiedeberg's Archives of Pharmacology. 297:275-281 |
| ISSN: | 1432-1912 0028-1298 |
| DOI: | 10.1007/bf00509272 |
| Popis: | The mechanism of the increase of phosphatidylcholine in liver, accompanying enzyme induction by phenobarbital, has been studied in rats. Using radioactively labeled precursors, the two main pathways of phosphatidylcholine biosynthesis--the CDP-choline pathway and the methylation of phosphatidylethanolamine--were analyzed after pretreatment with 4 doses of phenobarbital (80 mg/kg) on 3 consecutive days. After i.v. injection of choline [Me-3H], choline [Me-14C] or NaH2[32P]O4 the specific radioactivity (sp. act.) of phosphatidylcholine (dpm/nmol) was decreased by 60%, and after methionine [Me-3H] or ethanolamine [1.2-14C] by 40% compared to control rats. These changes are partly due to the increased concentration of phosphatidylcholine and phosphatidylethanolamine, causing the incorporated precursors to dilute, and partly to a secondary effect which leads to a reduction of the sp. act. of free choline in pretreated animals. The concentration of glycerylphosphorylcholine, one of the metabolites of phosphatidylcholine catabolism, was also diminished by almost 50%. From these results it may be concluded that the increase of phosphatidylcholine is due to a retardation of its breakdown rather than to an increase of its synthesis. |
| Databáze: | OpenAIRE |
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