Proteomics in Drosophila melanogaster: first 2D database of larval hemolymph proteins
| Autor: | Liliane Schoofs, Arnold De Loof, Geert Baggerman, Anja Cerstiaens, Evy Vierstraete, Gert Van den Bergh |
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| Rok vydání: | 2003 |
| Předmět: |
Proteomics
Biophysics Tandem mass spectrometry Biochemistry Peptide mass fingerprinting Hemolymph Animals Drosophila Proteins Humans Electrophoresis Gel Two-Dimensional Databases Protein Molecular Biology Polyacrylamide gel electrophoresis Gel electrophoresis Chromatography biology fungi Blood Proteins Cell Biology biology.organism_classification Drosophila melanogaster Larva Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Proteome Isoelectric Focusing |
| Zdroj: | Biochemical and Biophysical Research Communications. 304:831-838 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/s0006-291x(03)00683-1 |
| Popis: | A proteomic approach was used for the identification of larval hemolymph proteins of Drosophila melanogaster. We report the initial establishment of a two-dimensional gel electrophoresis reference map for hemolymph proteins of third instar larvae of D. melanogaster. We used immobilized pH gradients of pH 4-7 (linear) and a 12-14% linear gradient polyacrylamide gel. The protein spots were silver-stained and analyzed by nanoLC-Q-Tof MS/MS (on-line nanoscale liquid chromatography quadrupole time of flight tandem mass spectrometry) or by Matrix assisted laser desorption time of flight MS (MALDI-TOF MS). Querying the SWISSPROT database with the mass spectrometric data yielded the identity of the proteins in the spots. The presented proteome map lists those protein spots identified to date. This map will be updated continuously and will serve as a reference database for investigators, studying changes at the protein level in different physiological conditions. |
| Databáze: | OpenAIRE |
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