secA gene suitability for fast and easy identification of Phytoplasmas by RFLP analysis
| Autor: | M. Zizyte, D. Valiunas, Donatas Sneideris, Algirdas Ivanauskas |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
0301 basic medicine Genetics biology Rare cutting nucleases Strain (biology) Single step Plant Science Horticulture 16S ribosomal RNA biology.organism_classification 01 natural sciences 03 medical and health sciences Restriction enzyme 030104 developmental biology Phytoplasma identification Phytoplasma RFLP Primer (molecular biology) Restriction fragment length polymorphism Agronomy and Crop Science Gene secA 010606 plant biology & botany |
| Zdroj: | European Journal of Plant Pathology. 160:737-743 |
| ISSN: | 1573-8469 0929-1873 |
| Popis: | n this study we investigated the secA gene as possible marker to supplement the classification scheme based on 16S rRNA sequences. We cloned and sequenced secA gene and its flanking region of 'Ca. P. asteris' CPh strain phytoplasma, as well as 1997 bp length secA gene fragment of Canada peach X-disease CX and 1327 bp fragment of 'Ca. P. ziziphi' JWB. This helped us to select a primer pair for a single step PCR that can amplify similar to 1200 kb length fragment of secA gene from different phytoplasma groups. We propose that this fragment could be used in RFLP analysis to quickly identify and distinguish 16SrI-A, I-B, I-C, I-D, III-A, III-B, III-E, III-F, III-H, V-B, XII-B and XXI-A subgroup phytoplasmas using just two restriction endonucleases. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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