Photosynthetic production of glycerol by a recombinant cyanobacterium
| Autor: | Klaas J. Hellingwerf, Wei Du, Philipp Savakis, Filipe Branco dos Santos, Xiaoming Tan, Xuefeng Lu |
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| Přispěvatelé: | Molecular Microbial Physiology (SILS, FNWI) |
| Rok vydání: | 2014 |
| Předmět: |
Cyanobacteria
Glycerol biology Synechocystis Saccharomyces cerevisiae Mutant DNA Recombinant Bioengineering General Medicine Sodium Chloride biology.organism_classification Photosynthesis Applied Microbiology and Biotechnology Metabolic engineering chemistry.chemical_compound chemistry Biochemistry Metabolic Engineering Osmotic Pressure Mutation Extracellular Biotechnology |
| Zdroj: | Journal of Biotechnology, 195, 46-51. Elsevier |
| ISSN: | 1873-4863 0168-1656 |
| Popis: | Cyanobacteria are prokaryotic organisms capable of oxygenic photosynthesis. Glycerol is an important commodity chemical. Introduction of phosphoglycerol phosphatase 2 from Saccharomyces cerevisiae into the model cyanobacterium Synechocystis sp. PCC6803 resulted in a mutant strain that produced a considerable amount of glycerol from light, water and CO2. Mild salt stress (200 mM NaCl) on the cells led to an increase of the extracellular glycerol concentration of more than 20%. Under these conditions the mutant accumulated glycerol to an extracellular concentration of 14.3 mM after 17 days of culturing. (C) 2014 Elsevier B.V. All rights reserved. |
| Databáze: | OpenAIRE |
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