Polyoma Pseudovirions I. Sequence of Events in Primary Mouse Embryo Cells Leading to Pseudovirus Production
| Autor: | David B. Yelton, H. Vasken Aposhian |
|---|---|
| Rok vydání: | 1972 |
| Předmět: |
DNA Replication
Time Factors viruses Immunology Biology Tritium Virus Replication Microbiology Virus Mice Viral Proteins chemistry.chemical_compound Nucleic acid thermodynamics Virology Animal Viruses Centrifugation Density Gradient Animals neoplasms Cells Cultured DNA replication Nucleic Acid Hybridization DNA biochemical phenomena metabolism and nutrition Molecular biology Molecular Weight Capsid Viral replication chemistry Insect Science embryonic structures DNA Viral Polyomavirus Thymidine Intracellular |
| Zdroj: | Journal of Virology. 10:340-346 |
| ISSN: | 1098-5514 0022-538X |
| DOI: | 10.1128/jvi.10.3.340-346.1972 |
| Popis: | The relationship of the intracellular events leading to the production of polyoma pseudovirions in primary mouse embryo cells has been investigated. Replication of polyoma deoxyribonucleic acid (DNA) began 18 hr after infection. Assembly of viral capsid protein occurred 12 hr later. Intracellular fragments of host cell DNA, of the size found in pseudovirions, were first detected 36 hr after infection. The amount of intracellular 14 S host DNA that was produced during infection was seven times greater than the amount of polyoma DNA synthesized. The relative pool sizes of polyoma DNA and 14 S DNA at the time of virus assembly may dictate the amounts of polyoma virus and pseudovirus produced. |
| Databáze: | OpenAIRE |
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