Contributions of single-cell genomics to our understanding of planktonic marine archaea
| Autor: | Sarah M. Laperriere, Matthew Kellom, Alyson E. Santoro |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Metabolic function
biology Ecology (disciplines) Group ii Genomics Computational biology Population biology Articles Plankton biology.organism_classification Genome Archaea General Biochemistry Genetics and Molecular Biology Genome Archaeal Single-Cell Analysis General Agricultural and Biological Sciences |
| Zdroj: | Philos Trans R Soc Lond B Biol Sci |
| ISSN: | 1471-2970 |
| Popis: | Single-cell genomics has transformed many fields of biology, marine microbiology included. Here, we consider the impact of single-cell genomics on a specific group of marine microbes—the planktonic marine archaea. Despite single-cell enabled discoveries of novel metabolic function in the marine thaumarchaea, population-level investigations are hindered by an overall lower than expected recovery of thaumarchaea in single-cell studies. Metagenome-assembled genomes have so far been a more useful method for accessing genome-resolved insights into the Marine Group II euryarchaea. Future progress in the application of single-cell genomics to archaeal biology in the ocean would benefit from more targeted sorting approaches, and a more systematic investigation of potential biases against archaea in single-cell workflows including cell lysis, genome amplification and genome screening. This article is part of a discussion meeting issue ‘Single cell ecology’. |
| Databáze: | OpenAIRE |
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