Synergistic effect of CNTF and GDNF on directed neurite growth in chick embryo dorsal root ganglia

Autor: Ji Hyun Kim, Michael W. Kim, Young Min Ju, Aurelia Poerio, Vladimir S. Mashanov, Dehui Lai, James J. Yoo, Abdelrahman Alwan
Jazyk: angličtina
Rok vydání: 2020
Předmět:
0301 basic medicine
Chick Embryo
Ciliary neurotrophic factor
Nervous System
Biochemistry
Signaling Molecules
Tissue culture
0302 clinical medicine
Cell Signaling
Animal Cells
Neurotrophic factors
Ganglia
Spinal
Medicine and Health Sciences
Morphogenesis
Glial cell line-derived neurotrophic factor
Nerve Tissue
Materials
Cells
Cultured
Neurons
Multidisciplinary
biology
Chemistry
Chemotaxis
Ganglion
Cell biology
Cell Motility
medicine.anatomical_structure
Physical Sciences
Medicine
Biological Cultures
Cellular Types
Anatomy
Research Article
Signal Transduction
Cell signaling
Neurite
Amorphous Solids
Neurogenesis
Science
Materials Science
Research and Analysis Methods
03 medical and health sciences
Neurites
medicine
Regeneration
Animals
Ciliary Neurotrophic Factor
Glial Cell Line-Derived Neurotrophic Factor
Tissue Cultures
Biology and Life Sciences
Proteins
Cell Biology
Neuronal Dendrites
Nerve Regeneration
Biological Tissue
030104 developmental biology
Cellular Neuroscience
Mixtures
biology.protein
Ganglia
Gels
Collagens
Organism Development
030217 neurology & neurosurgery
Neuroscience
Developmental Biology
Zdroj: PLoS ONE, Vol 15, Iss 10, p e0240235 (2020)
PLoS ONE
ISSN: 1932-6203
Popis: It is often critical to improve the limited regenerative capacity of the peripheral nerves and direct neural growth towards specific targets, such as surgically implanted bioengineered constructs. One approach to accomplish this goal is to use extrinsic neurotrophic factors. The candidate factors first need to be identified and characterized in in vitro tests for their ability to direct the neurite growth. Here, we present a simple guidance assay that allows to assess the chemotactic effect of signaling molecules on the growth of neuronal processes from dorsal root ganglia (DRG) using only standard tissue culture materials. We used this technique to quantitatively determine the combined and individual effects of the ciliary neurotrophic factor (CNTF) and glial cell line-derived neurotrophic factor (GDNF) on neurite outgrowth. We demonstrated that these two neurotrophic factors, when applied in a 1:1 combination, but not individually, induced directed growth of neuronal processes towards the source of the gradient. This chemotactic effect persists without significant changes over a wide (10-fold) concentration range. Moreover, we demonstrated that other, more general growth parameters that do not evaluate growth in a specific direction (such as, neurite length and trajectory) were differentially affected by the concentration of the CNTF/GNDF mixture. Furthermore, GDNF, when applied individually, did not have any chemotactic effect, but caused significant neurite elongation and an increase in the number of neurites per ganglion.
Databáze: OpenAIRE
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