Cell-Directed Assembly of Lipid-Silica Nanostructures Providing Extended Cell Viability
| Autor: | Zhu Chen, Helen K. Baca, Susan M. Brozik, Eric C. Carnes, Hongyou Fan, Margaret Werner-Washburne, Seema Singh, Gabriel P. Lopez, DeAnna M. Lopez, Darren R. Dunphy, Jeb H. Flemming, Carlee Erin Ashley, Nanguo Liu, C. Jeffrey Brinker |
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| Rok vydání: | 2006 |
| Předmět: |
Silicon dioxide
Green Fluorescent Proteins Lipid Bilayers Cell Saccharomyces cerevisiae Buffers Bacterial cell structure Cell membrane chemistry.chemical_compound Amphiphile Escherichia coli medicine Scattering Radiation Viability assay Lipid bilayer Micelles Phospholipids Multidisciplinary X-Rays Cell Membrane Fluorescence recovery after photobleaching Cells Immobilized Hydrogen-Ion Concentration Silicon Dioxide Recombinant Proteins Nanostructures Cell biology Microscopy Electron medicine.anatomical_structure chemistry Biophysics Bacillus subtilis Fluorescence Recovery After Photobleaching |
| Zdroj: | Science. 313:337-341 |
| ISSN: | 1095-9203 0036-8075 |
| Popis: | Amphiphilic phospholipids were used to direct the formation of biocompatible, uniform silica nanostructures in the presence of Saccharomyces cerevisiae and bacterial cell lines. The cell surfaces organize multilayered phospholipid vesicles that interface coherently with the silica host and help relieve drying stresses that develop with conventional templates. These host structures maintain cell accessibility, addressability, and viability in the absence of buffer or an external fluidic architecture. The cell surfaces are accessible and can be used to localize added proteins, plasmids, and nanocrystals. Prolonged cell viability combined with reporter protein expression enabled stand-alone cell-based sensing. |
| Databáze: | OpenAIRE |
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