MTS-conjugated-antiactive caspase 3 antibodies inhibit actinomycin D-induced apoptosis
| Autor: | Yang Zhao, Heinz Kohler, Tom Brown, Sybille Muller |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Cancer Research
Programmed cell death Clinical Biochemistry Pharmaceutical Science Apoptosis Caspase 3 DNA Fragmentation Antibodies Jurkat Cells Humans Caspase Pharmacology chemistry.chemical_classification biology Biochemistry (medical) Spectrin Cell Biology Molecular biology Enzyme chemistry Polyclonal antibodies Caspases Monoclonal Dactinomycin biology.protein Antibody Cell Division |
| Zdroj: | Apoptosis. 8:631-637 |
| ISSN: | 1360-8185 |
| DOI: | 10.1023/a:1026139627930 |
| Popis: | Caspase 3 is critically involved in the pathway of apoptosis. We have conjugated a MTS-transport-peptide to monoclonal and polyclonal anti-caspase-3 antibodies to suppress Actinomycin D-induced apoptosis in human lymphoma T cells. The advantage of using trans-membrane antibodies compared to conventional apoptosis inhibitors is their specific target recognition in the living cell and their lower toxicity compared to conventional apoptosis inhibitors. We could show that a MTS-transport-peptide modified monoclonal anti-caspase-3 antibody reduces Actinomycin D induced apoptosis, as shown by DNA ladder electrophoresis and cell death ELISA. These results indicate that antibodies have a therapeutic potential to inhibit apoptosis in a variety of diseases. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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