Generalized spectral phase-only time-domain ptychographic phase reconstruction applied in nonlinear microscopy
| Autor: | Thomas Feurer, Alexander M. Heidt, Pieter Neethling, Gurthwin Bosman, Erich G. Rohwer, Dirk Spangenberg, George Dwapanyin |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Materials science
530 Physics business.industry Phase (waves) FOS: Physical sciences Statistical and Nonlinear Physics 620 Engineering 01 natural sciences Atomic and Molecular Physics and Optics Ptychography Supercontinuum 010309 optics Optics Pulse compression Compression (functional analysis) 0103 physical sciences Time domain business Excitation Photonic-crystal fiber Optics (physics.optics) Physics - Optics |
| Popis: | Nonlinear microscopy has evolved over the last few decades to become a powerful tool for imaging and spectroscopic applications in biological sciences. In this study, I$^2$PIE, a novel spectral phase control technique, was implemented in order to compress broad-bandwidth supercontinuum light pulses generated in an all-normal-dispersion (ANDi) photonic crystal fiber (PCF). The technique, based on time-domain ptychography, is demonstrated here in a nonlinear microscopy application for the first time, to the best of our knowledge. The first real-world application of this technique for second-harmonic generation and two-photon excitation fluorescence microscopies in biological samples is presented. We further show that in our implementation, I$^2$PIE leads to improved contrast and signal-to-noise ratios in the generated images, compared to conventional compression techniques used in nonlinear microscopy. Copyright 2020 Optical Society of America. One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modifications of the content of this paper are prohibited |
| Databáze: | OpenAIRE |
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