Assessing the DNA Damaging Effectiveness of Ionizing Radiation Using Plasmid DNA
| Autor: | Yara Maayah, Humza Nusrat, Geordi Pang, Mauro Tambasco |
|---|---|
| Rok vydání: | 2022 |
| Předmět: |
Organic Chemistry
Dose-Response Relationship Radiation DNA General Medicine Catalysis Computer Science Applications Inorganic Chemistry Radiation Ionizing DNA-based detector X-ray ionizing radiation DNA DSB damage oxidized DNA base damage linear energy transfer relative biological effectiveness Physical and Theoretical Chemistry Molecular Biology Spectroscopy Aluminum DNA Damage Plasmids |
| Zdroj: | International Journal of Molecular Sciences; Volume 23; Issue 20; Pages: 12459 |
| ISSN: | 1422-0067 |
| DOI: | 10.3390/ijms232012459 |
| Popis: | Plasmid DNA is useful for investigating the DNA damaging effects of ionizing radiation. In this study, we have explored the feasibility of plasmid DNA-based detectors to assess the DNA damaging effectiveness of two radiotherapy X-ray beam qualities after undergoing return shipment of ~8000 km between two institutions. The detectors consisted of 18amp;nbsp;μL of pBR322 DNA enclosed with an aluminum seal in nine cylindrical cavities drilled into polycarbonate blocks. We shipped them to Toronto, Canada for irradiation with either 100amp;nbsp;kVpamp;nbsp;or 6amp;nbsp;MVamp;nbsp; X-ray beams to doses of 10, 20, and 30 Gy in triplicate before being shipped back to San Diego, USA. The Toronto return shipment also included non-irradiated controls and we kept a separate set of controls in San Diego. In San Diego, we quantified DNA single strand breaks (SSBs), double strand breaks (DSBs), and applied Nth and Fpg enzymes to quantify oxidized base damage. The rate of DSBs/Gy/plasmid was 2.8±0.7amp;nbsp;greater for the 100 kVp than the 6 MV irradiation. The 100amp;nbsp;kVp irradiation also resulted in 5±2 times more DSBs/SSB than the 6amp;nbsp;MV beam, demonstrating that the detector is sensitive enough to quantify relative DNA damage effectiveness, even after shipment over thousands of kilometers. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
| Nepřihlášeným uživatelům se plný text nezobrazuje | K zobrazení výsledku je třeba se přihlásit. |