TgVTC2 is involved in polyphosphate accumulation in Toxoplasma gondii
| Autor: | Lawrence Ayong, Crystal M. Tobin, Silvia N.J. Moreno, Laura J. Knoll, Peggy J. Rooney |
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| Rok vydání: | 2010 |
| Předmět: |
Mutant
Saccharomyces cerevisiae Protozoan Proteins Locus (genetics) Biology Article Polyphosphates Cloning Molecular Molecular Biology Genetic Association Studies Kinase Genetic Complementation Test Toxoplasma gondii biology.organism_classification Molecular biology Protein Structure Tertiary Up-Regulation Complementation Transmembrane domain Mutagenesis Insertional Cytoplasm Parasitology Protein Kinases Toxoplasma Gene Deletion Toxoplasmosis Molecular Chaperones |
| Zdroj: | Molecular and biochemical parasitology. 176(2) |
| ISSN: | 1872-9428 |
| Popis: | Polyphosphate is found in every cell, having roles in diverse processes, including differentiation and response to stress. In this study, we characterize a Toxoplasma gondii mutant containing an insertion within the carboxy-terminal end of a homolog of Saccharomyces cerevisiae Vtc2p, a component of the polyphosphate synthetic machinery. Locus TgVTC2 encodes a 140 kDa protein containing conserved SPX, VTC and transmembrane domains. TgVTC2 localizes in punctate spots within the cytoplasm that do not co-localize with known markers. The TgVTC2 mutant showed dramatically reduced polyphosphate accumulation, a defect restored by introduction of TgVTC2 to the mutant. Insertion within TgVTC2 resulted in increased transcript levels for two loci, including a putative FIKK kinase. These transcript levels were restored to wild-type levels upon complementation with the TgVTC2 locus. The TgVTC2 locus was refractory to knockout, and may be essential. Analysis of this TgVTC2 mutant will facilitate dissection of the T. gondii polyphosphate synthesis pathway. |
| Databáze: | OpenAIRE |
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