Specific Determination of Proline in Biological Materials

Autor:	Arthur E. Pasieka, Joseph F. Morgan
Rok vydání:	1956
Předmět:	Absorption (pharmacology) chemistry.chemical_classification Chromatography Proline Chemistry Reagent Dilute acid Indicators and Reagents General Biochemistry Genetics and Molecular Biology Quantitative determination Biological materials Amino acid
Zdroj:	Experimental Biology and Medicine. 93:54-57
ISSN:	1535-3699 1535-3702
DOI:	10.3181/00379727-93-22661
Popis:	SummaryA specific method for determining proline is reported, based on treatment of isatin-developed paper chromatograms with dilute acid. None of the 20 other amino acids of synthetic medium M 150, including hy-droxyproline, reacted by this procedure. The proline chromogen exhibits an absorption maximum at 600 mμ. The color is remarkably stable and can be used for the quantitative determination of proline over the range of 1 to 16 μg.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c6b9e5f488ec8d325c12cc426f3b856b https://doi.org/10.3181/00379727-93-22661 Zobrazit plný text záznamu