Longitudinal Analysis of Gene Expression in Porcine Skeletal Muscle After Post-Injection Local Injury
| Autor: | Pierre-Louis Toutain, François Hatey, Pierre J. Ferré, Magali SanCristobal, Agnès Bonnet, Gwenola Tosser-Klopp, Laurence Liaubet, Didier Concordet, Emmanuelle Uro-Coste, Hervé P. Lefebvre |
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| Přispěvatelé: | Physiologie et Toxicologie Expérimentales, Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Laboratoire de Génétique Cellulaire (LGC), Régulations cellulaires: lipidoses et atherosclerose, IFR 31 Louis Bugnard (IFR 31), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Toulouse [Toulouse]-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Toulouse [Toulouse]-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM), Simon, Marie Francoise, Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre Hospitalier Universitaire de Toulouse (CHU Toulouse)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre Hospitalier Universitaire de Toulouse (CHU Toulouse)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM), Ecole Nationale Vétérinaire de Toulouse (ENVT), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Recherche Agronomique (INRA), Régulations cellulaires: lipidoses et atherosclerose. |
| Rok vydání: | 2007 |
| Předmět: |
Male
Pathology Time Factors MESH: Thymosin Swine [SDV]Life Sciences [q-bio] Muscle Proteins MESH: Collagen Type I Pharmaceutical Science Post injection MESH: Down-Regulation MESH: Collagen MESH: Fibronectins Gene expression MESH: Up-Regulation Osteonectin MESH: Animals Pharmacology (medical) MESH: Swine Oligonucleotide Array Sequence Analysis MESH: Muscle Skeletal 0303 health sciences 030302 biochemistry & molecular biology MESH: Injections Intramuscular Pathophysiology Up-Regulation [SDV] Life Sciences [q-bio] medicine.anatomical_structure Matrix Metalloproteinase 2 Molecular Medicine Collagen MESH: Osteonectin Intramuscular injection MESH: ras Proteins Biotechnology medicine.medical_specialty Down-Regulation Biology Injections Intramuscular Collagen Type I MESH: Gene Expression Profiling MESH: Muscle Proteins 03 medical and health sciences medicine Animals Muscle Skeletal Pathological 030304 developmental biology Pharmacology Gene Expression Profiling MESH: Time Factors Organic Chemistry Skeletal muscle MESH: Male Fibronectins Collagen Type I alpha 1 Chain Thymosin MESH: Matrix Metalloproteinase 2 Gene expression profiling MESH: Oligonucleotide Array Sequence Analysis Time course ras Proteins |
| Zdroj: | Pharmaceutical Research Pharmaceutical Research, American Association of Pharmaceutical Scientists, 2007, 24 (8), pp.1480-9. ⟨10.1007/s11095-007-9266-8⟩ Pharmaceutical Research, 2007, 24 (8), pp.1480-9. ⟨10.1007/s11095-007-9266-8⟩ Pharmaceutical Research / Pharmaceutical Research (Dordrecht) Pharmaceutical Research / Pharmaceutical Research (Dordrecht), 2007, 24 (8), pp.1480-9. ⟨10.1007/s11095-007-9266-8⟩ |
| ISSN: | 1573-904X 0724-8741 |
| DOI: | 10.1007/s11095-007-9266-8 |
| Popis: | International audience; PURPOSE: The purpose of this study is to describe the time course of gene expression in a skeletal muscle local injury induced by an intramuscular (IM) injection, and to compare the dynamics of gene expression with pathological events. MATERIALS AND METHODS: Ten piglets received 4 IM injections of propylene glycol in the longissimus dorsi muscles 6 h, 2, 7, and 21 days before euthanasia, where control and injected muscle sites were sampled for RNA isolation and microscopic examination. The hybridization of nylon cDNA microarrays was carried out with radioactive probes obtained from the muscle RNA. RESULTS: 153 genes were found under- or over-expressed at least once among the investigated time-conditions. The eight most discriminant genes were also identified: Two genes (GTP-binding protein RAD and Ankyrin repeat domain protein) were over-expressed at 6 h and six genes between 2 and 21 days (Osteonectin, Fibronectin, Matrix metalloproteinase-2, Collagen alpha 1(I) chain, Collagen alpha 2(I) chain, and Thymosin beta-4). Necrosis, inflammation and regeneration were observed through both the dynamics of gene expression profiles and through the microscopic examinations. CONCLUSION: Our data demonstrate that several pathways are involved in post-injection muscle injury, and that necrosis, inflammation and regeneration are not sequential but occur in parallel. |
| Databáze: | OpenAIRE |
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