Does Biliodigestive Anastomosis Have Any Effect on the Reversal of Hepatopulmonary Syndrome in a Biliary Cirrhosis Experimental Model?
| Autor: | Ana Cristina Aoun Tannuri, Vitor Ribeiro Paes, Maria Cecília Mendonça Coelho, Suellen Serafini, Karina Miura da Costa, Leonardo Ervolino Corbi, Rebeca Lopes Figueira, Josiane de Oliveira Gonçalves, Lourenço Sbragia, Uenis Tannuri, Maria Julia de Aro Braz |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Male
medicine.medical_specialty Physiology Biliary cirrhosis Anastomosis Gastroenterology Hypoxemia 03 medical and health sciences 0302 clinical medicine Internal medicine medicine Animals Rats Wistar Hepatopulmonary syndrome Ligation Lung Liver Cirrhosis Biliary business.industry Anastomosis Surgical Hepatology medicine.disease Pathophysiology Rats Disease Models Animal 030220 oncology & carcinogenesis Arterial blood Female 030211 gastroenterology & hepatology Bile Ducts medicine.symptom business Endothelin receptor Hepatopulmonary Syndrome |
| Zdroj: | Digestive Diseases and Sciences. 64:3192-3202 |
| ISSN: | 1573-2568 0163-2116 |
| DOI: | 10.1007/s10620-019-05658-8 |
| Popis: | Biliary cirrhosis is associated with hepatopulmonary syndrome (HPS), which is related to increased posttransplant morbidity and mortality. This study aims to analyze the pathophysiology of biliary cirrhosis and the onset of HPS. Twenty-one-day-old Wistar rats were subjected to common bile duct ligation and were allocated to two groups: group A (killed 2, 3, 4, 5, or 6 weeks after biliary obstruction) and group B (subjected to biliodigestive anastomosis 2, 3, 4, 5, or 6 weeks after the first procedure and killed 3 weeks later). At the killing, arterial blood was collected for the analyses, and samples from the liver and lungs were collected for histologic and molecular analyses. The gasometric parameters as well as the expression levels of ET-1, eNOS, and NOS genes in the lung tissue were evaluated. From a total of 42 blood samples, 15 showed hypoxemia (pO2 18 mmHg]. The liver histology revealed increased ductular proliferation after common bile duct ligation, and reconstruction of bile flow promoted decreased ductular proliferation 5 and 6 weeks post-common bile duct ligation. Pulmonary alterations consisted of decreased parenchymal airspace and increased medial wall thickness. Biliary desobstruction promoted transitory improvements 5 weeks after biliary obstruction (increased parenchymal airspace and decreased MWT—p = 0.003 and p = 0.004, respectively) as well as increased endothelin expression levels (p = 0.009). The present model showed lung tissue alterations promoted by biliary obstruction. The biliodigestive anastomosis had no clear direct effects on these alterations. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink