Lipid plasma concentrations of HDL subclasses determined by enzymatic staining on polyacrylamide electrophoresis gels in children with metabolic syndrome
| Autor: | David Cruz-Robles, Oscar Pérez-Méndez, Gilberto Vargas-Alarcón, Minerva Juárez-Meavepeña, Cynthia García-Sánchez, Mariana Monter-Garrido, Margarita Torres-Tamayo, Cristhel López-Osorio, Paola Toledo-Ibelles, Elizabeth Carreón-Torres |
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| Rok vydání: | 2011 |
| Předmět: |
Male
medicine.medical_specialty Surface Properties Clinical Biochemistry Biochemistry chemistry.chemical_compound Phosphatidylcholine Internal medicine medicine Humans Distribution (pharmacology) Child Triglycerides Metabolic Syndrome chemistry.chemical_classification Cholesterol Biochemistry (medical) nutritional and metabolic diseases General Medicine medicine.disease Enzymes Staining Endocrinology Enzyme chemistry Case-Control Studies Phosphatidylcholines Electrophoresis Polyacrylamide Gel Female lipids (amino acids peptides and proteins) Composition (visual arts) Metabolic syndrome Lipoproteins HDL Dyslipidemia |
| Zdroj: | Clinica Chimica Acta. 412:292-298 |
| ISSN: | 0009-8981 |
| DOI: | 10.1016/j.cca.2010.10.021 |
| Popis: | Background The antiatherogenic role of different HDL subclasses is still controversial. HDL particles of the same size can have different lipid contents in some physiopathological situations. However, little is known about the plasma lipid levels of HDL subclasses when they are separated by their hydrodynamic diameter. Methods Triglycerides (Tg), phosphatidylcholine (Ph), and cholesterol (C) plasma concentrations of HDL subclasses, were determined by enzymatic staining on polyacrylamide gradient gel (PAGE) in 50 pediatric patients with metabolic syndrome (MS), and 50 control children paired by age and gender. Proteins of HDL subclasses were also stained for the assessment of the relative size distribution of HDL. Results Relative HDL size distribution was shifted to small particles in MS pediatric patients when determined per protein. In contrast, cholesterol plasma concentrations corresponding to the HDL2b, 2a, 3a, and 3b subclasses were decreased; triglycerides of HDL3b and 3c, as well as plasma phospholipids from HDL3c, were elevated in MS patients as compared to controls. The C-to-Ph ratio, considered as indicative of HDL composition, was similar among the 5 HDL subclasses in control subjects, whereas this ratio gradually decreased from large HDL2b to small HDL3c in the MS group. Cholesterol plasma concentrations of HDL subclasses correlated with the components of the MS. Conclusions Lipids of HDL subclasses provide more and accurate information than the relative HDL size distribution determined by protein staining, and may contribute to understand better HDL metabolism and the coronary risk associated to these lipoproteins. |
| Databáze: | OpenAIRE |
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