Apoptosis-Inducing Effect of Fucoxanthin on Human Leukemia Cell Line HL-60
| Autor: | Kozue Miyauchi, Hiroyuki Kohno, Koretaro Takahashi, Satoshi Wanezaki, Jun Kawabata, Shizuo Odashima, Hideyuki Kurihara, Masashi Hosokawa |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1999 |
| Předmět: |
Marketing
antiproliferation General Chemical Engineering apoptosis DNA fragmentation Cell cycle Biology Molecular biology Industrial and Manufacturing Engineering Incubation period fucoxanthin chemistry.chemical_compound Biochemistry chemistry Cell culture Apoptosis Agarose gel electrophoresis Fucoxanthin HL-60 cell Incubation Food Science Biotechnology |
| Zdroj: | Food Science and Technology Research. 5(3):243-246 |
| ISSN: | 1344-6606 |
| Popis: | Note The apoptosis-inducing effect of fucoxanthin on human leukemic HL-60 cells was investigated. Fucoxanthin, which was obtained from the brown alga Undaria pinnatifida, inhibited the proliferation of HL-60 cells and induced DNA fragmentation on agarose gel electrophoresis, a typical characteristic feature of apoptotic cells. The results of sandwich ELISA using anti-biotin-antibody and anti-DNA-antibody also demonstrated DNA fragmentation in accordance with the increase in fucoxanthin concentration and incubation time. Since the level of DNA fragmentation did not increase after 24 h incubation, fucoxanthin appeared to have some effect on cell cycle. In contrast, β-carotene did not show an apoptosis-inducing effect on HL-60 cells. These results suggested that a carotenoid structure might be crucial for inducing apoptosis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|