Wound Healing Effect of Conditioned Media Obtained From Adipose Tissue on Human Skin Cells
| Autor: | Hendrik Jan Ankersmit, Alfred Gugerell, Maike Keck, Stefan Hacker, Johanna Kober, Maximilian Zeyda, Elisabeth Buchberger, Stefanie Nickl, Melanie Schmid |
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| Rok vydání: | 2016 |
| Předmět: |
Keratinocytes
0301 basic medicine Pathology medicine.medical_specialty Angiogenesis Cellular differentiation medicine.medical_treatment Cell Subcutaneous Fat Neovascularization Physiologic Adipose tissue Human skin In Vitro Techniques 03 medical and health sciences Cell Movement Adipocytes medicine Humans Cell Proliferation Wound Healing business.industry Cell Differentiation Epithelial Cells Mesenchymal Stem Cells Fibroblasts 030104 developmental biology medicine.anatomical_structure Culture Media Conditioned Skin grafting Surgery Stem cell Wound healing business |
| Zdroj: | Annals of Plastic Surgery. 77:156-163 |
| ISSN: | 0148-7043 |
| Popis: | BACKGROUND Split-thickness skin grafting is the gold standard to cover extensive acute and chronic wounds with a well-vascularized wound bed. Although some headway has been made in developing biological agents to speed up healing, there is still no treatment that sufficiently replaces skin grafts to date. The use of secretory factors of adipose tissue may be a feasible approach to developing topical wound applications for faster wound healing. METHODS In this study, the effect of conditioned media (CMs) of human adipose-derived stem cells (ASCs), adipocytes, or adipose tissue on human skin cells was evaluated for viability, proliferation, and migration in vitro. Differentiation potential of stem cells treated with CM was monitored by AdipoRed staining and qualitative real-time polymerase chain reaction. Angiogenic potential of human endothelial cells treated with CM was tested via sprouting assay. RESULTS The CM of adipose tissue significantly enhanced ASC proliferation (P < 0.01). Treatment with CM showed no inductive effect on ASC differentiation into adipocytes but, at the same time, significantly induced cell sprouting of endothelial cells (P < 0.001). We show for the first time that CM of adipose tissue is a potent inducer of proliferation of ASCs and angiogenesis, with comparable effects with those of stem cell-enriched CM. CONCLUSIONS We suggest the use of the secretome of adipose tissue to produce CM for topical application on wounds, rather than working with adipose tissue or including the difficult process of enriching the patients' stem cells in vitro. |
| Databáze: | OpenAIRE |
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