Atrophy of mesenteric sympathetic innervation may contribute to splanchnic vasodilation in rat portal hypertension
| Autor: | Rafael Esteban, Javier Hernández-Losa, Joan Genescà, Jaume Bosch, María Martell, Imma Raurell, Silvia Cuenca, Jaume Guardia, Nahia Ezkurdia, Mar Coll |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Male
medicine.medical_specialty Sympathetic Nervous System Down-Regulation Adrenergic Vasodilation Statistics Nonparametric Rats Sprague-Dawley Random Allocation Nerve Fibers Mesenteric Artery Superior Internal medicine medicine.artery Hypertension Portal Animals Medicine Mesentery RNA Messenger Splanchnic Circulation Superior mesenteric artery Mesenteric arteries Hepatology Tyrosine hydroxylase business.industry Hemodynamics medicine.disease SMA Immunohistochemistry Rats Disease Models Animal Endocrinology medicine.anatomical_structure Portal hypertension Atrophy business Splanchnic |
| Zdroj: | Liver International. 30:593-602 |
| ISSN: | 1478-3231 1478-3223 |
| Popis: | Background and aims: Portal hypertension is associated with downregulation of mRNA and proteins involved in adrenergic transmission in the superior mesenteric artery (SMA) in portal vein-ligated (PVL) and cirrhotic rats. We aimed to investigate whether SMA adrenergic dysfunction was accompanied by sympathetic nerve structural changes and whether it was extensive to resistance mesenteric arteries. We also attempted to localize the origin of mRNA of specific adrenergic genes. Methods and results: In situ hybridization showed tyrosine hydroxylase (Th) mRNA expression in neuronal bodies of superior mesenteric ganglia and inside axonal fibres surrounding proximal SMA sections. Comparison of SMA by Th immunohistochemistry, both in PVL and bile duct-ligated (BDL) rats, demonstrated a significant decrease in the number of nervous structures (69% PVL; 62% BDL), total nervous area (70% PVL; 52% BDL) and Th-stained nervous area (89% PVL; 64% BDL) compared with sham rats. A strong correlation was detected between the Th-stained nervous area and the haemodynamic parameters, mainly with SMA resistance (r=0.9, P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|