Interleukin-23 is constitutively expressed in the human annulus in vivo and in vitro, and is up-regulated in vitro by TNF-α

Autor:	E. Marrero, Michael Cox, Helen E. Gruber, Edward Hanley
Rok vydání:	2019
Předmět:	0301 basic medicine Histology Gene Expression Intervertebral Disc Degeneration Proinflammatory cytokine 03 medical and health sciences 0302 clinical medicine Downregulation and upregulation In vivo Gene expression medicine Humans Receptor Intervertebral Disc Cells Cultured Annulus (mycology) 030102 biochemistry & molecular biology Chemistry Tumor Necrosis Factor-alpha Annulus Fibrosus Intervertebral disc General Medicine In vitro Cell biology Up-Regulation Medical Laboratory Technology medicine.anatomical_structure 030220 oncology & carcinogenesis Interleukin-23 Subunit p19 Cytokines Intervertebral Disc Displacement
Zdroj:	Biotechnichistochemistry : official publication of the Biological Stain Commission. 94(7)
ISSN:	1473-7760
Popis:	Interleukin-23 (IL-23, IL-23p19) is a proinflammatory cytokine in the IL-12-related family. Although inflammatory cells in herniated discs have been shown to contain IL-23, little is known about the presence and role of IL-23 in human disc cells. We analyzed disc specimens for IL-23 localization using immunohistochemistry in control, herniated and non-herniated discs from which annulus fibrosus (annulus) cells were isolated and cultured to identify IL-23 gene expression and production. Microarray analysis was used to assess the expression of IL-23 in disc tissue and in cells exposed to two proinflammatory cytokines, IL-1s and TNF-α. IL-23 was present in annulus cells at the protein level and its expression was up-regulated significantly in herniated compared to control disc tissue. Direct measurement of medium components confirmed production of IL-23 and its receptor, IL-23R, by annulus cells in vitro. Annulus cells in three-dimensional culture exposed to TNF-α, but not IL-1s, resulted in significant up-regulation of IL-23 expression compared to control cells. Our findings are evidence for the constitutive presence of IL-23 in the human disc and that its expression in vitro is modified by exposure to TNF-α.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c6b341cfe2280dc566b17efc400b9231 https://pubmed.ncbi.nlm.nih.gov/31537133 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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