Detoxification of Atrazine by Endophytic Streptomyces sp. Isolated from Sugarcane and Detection of Nontoxic Metabolite
| Autor: | Alexandra Christine Helena Frankland Sawaya, Natália Reiko Sato Miyasaka, Josiane A. Mesquini, Begoña Gimenez-Cassina Lopez, Valéria Maia de Oliveira |
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| Rok vydání: | 2015 |
| Předmět: |
Health
Toxicology and Mutagenesis Microorganism Metabolite Toxicology Streptomyces Microbiology Soil chemistry.chemical_compound Tandem Mass Spectrometry RNA Ribosomal 16S Botany Animals Atrazine Soil Microbiology Rhizosphere biology Agriculture General Medicine biology.organism_classification 16S ribosomal RNA Pollution Saccharum Biodegradation Environmental Daphnia chemistry Ecotoxicity Soil microbiology |
| Zdroj: | Bulletin of Environmental Contamination and Toxicology. 95:803-809 |
| ISSN: | 1432-0800 0007-4861 |
| DOI: | 10.1007/s00128-015-1673-7 |
| Popis: | Atrazine is still one of the most used agricultural pesticides worldwide and it has been recognized as a major contaminant of surface and ground water. The aims of this research were to isolate an endophytic microorganism from leaves of sugarcane, evaluate its ability to degrade atrazine, and investigate the formation of metabolites. By sequencing of the 16S rRNA gene, the endophytic isolate atz2 was identified as Streptomyces sp. The reduction in atrazine concentration by Streptomyces sp. atz2 was 98 % and UHPLC-MS/MS analyses showed the appearance of an unknown metabolite observed as m/z 311. Ecotoxicity tests with an aquatic organism, Daphnia similis, confirmed that this metabolite was nontoxic. This mechanism of detoxification of atrazine is different from the ones of other free-living microorganisms that inhabit the soil or rhizosphere. The results show new aspects of atrazine detoxification, highlighting a new role of endophytic bacteria in plants. |
| Databáze: | OpenAIRE |
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