Impact of Exposure of Methicillin-Resistant Staphylococcus aureus to Polyhexanide In Vitro and In Vivo

Autor: Jacques Schrenzel, Abdessalam Cherkaoui, Alex J. O'Neill, Patrice Francois, Caroline Landelle, A. S. Bayer, Adriana Renzoni, Damien Baud, Stéphan Juergen Harbarth, E. von Dach, Seydina M. Diene, E-J Bonetti, Christopher P. Randall, R. Gonzales, Wessam Abdelhady, Caroline Manzano
Přispěvatelé: Techniques pour l'Evaluation et la Modélisation des Actions de la Santé (TIMC-IMAG-ThEMAS), Techniques de l'Ingénierie Médicale et de la Complexité - Informatique, Mathématiques et Applications, Grenoble - UMR 5525 (TIMC-IMAG), Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Centre Hospitalier Universitaire [Grenoble] (CHU), Laboratory of Genetics (UPV ⁄ EHU), University of the Basque Country/Euskal Herriko Unibertsitatea (UPV/EHU), Instituto de Ciencias Nucleares [Mexico], Universidad Nacional Autónoma de México (UNAM), Laboratory for Atmospheric and Space Physics [Boulder] (LASP), University of Colorado [Boulder], Institute of Environmental Physics [Heidelberg] (IUP), Universität Heidelberg [Heidelberg], Laboratoire Hubert Curien [Saint Etienne] (LHC), Institut d'Optique Graduate School (IOGS)-Université Jean Monnet [Saint-Étienne] (UJM)-Centre National de la Recherche Scientifique (CNRS), Service infectious diseases, Hôpitaux Universitaires de Genève (HUG), Prévention et contrôle des infections (PCI )
Jazyk: angličtina
Rok vydání: 2017
Předmět:
0301 basic medicine
Methicillin-Resistant Staphylococcus aureus
medicine.drug_class
[SDV]Life Sciences [q-bio]
030106 microbiology
Antibiotics
Polyhexanide
Biguanides
Mupirocin
Microbial Sensitivity Tests
medicine.disease_cause
Microbiology
Epidemiology and Surveillance
03 medical and health sciences
chemistry.chemical_compound
Antiseptic
Bacterial Proteins
Daptomycin
Cell Wall
Drug Resistance
Bacterial
medicine
Humans
Pharmacology (medical)
ComputingMilieux_MISCELLANEOUS
Pharmacology
ddc:616
business.industry
Chlorhexidine
High-Throughput Nucleotide Sequencing
Staphylococcal Infections
Aminoacyltransferases
Methicillin-resistant Staphylococcus aureus
3. Good health
Anti-Bacterial Agents
Repressor Proteins
030104 developmental biology
Infectious Diseases
chemistry
Staphylococcus aureus
Anti-Infective Agents
Local
[SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie
business
medicine.drug
Zdroj: Antimicrobial Agents and Chemotherapy
Antimicrobial Agents and Chemotherapy, American Society for Microbiology, 2017, 61 (10)
Antimicrobial Agents and Chemotherapy, Vol. 61, No 10 (2017) pp. e00272-17
ISSN: 0066-4804
1098-6596
Popis: Methicillin-resistant Staphylococcus aureus (MRSA) resistant to decolonization agents such as mupirocin and chlorhexidine increases the need for development of alternative decolonization molecules. The absence of reported severe adverse reactions and bacterial resistance to polyhexanide makes it an excellent choice as a topical antiseptic. In the present study, we evaluated the in vitro and in vivo capacity to generate strains with reduced polyhexanide susceptibility and cross-resistance with chlorhexidine and/or antibiotics currently used in clinic. Here we report the in vitro emergence of reduced susceptibility to polyhexanide by prolonged stepwise exposure to low concentrations in broth culture. Reduced susceptibility to polyhexanide was associated with genomic changes in the mprF and purR genes and with concomitant decreased susceptibility to daptomycin and other cell wall-active antibiotics. However, the in vitro emergence of reduced susceptibility to polyhexanide did not result in cross-resistance to chlorhexidine. During in vivo polyhexanide clinical decolonization treatment, neither reduced polyhexanide susceptibility nor chlorhexidine cross-resistance was observed. Together, these observations suggest that polyhexanide could be used safely for decolonization of carriers of chlorhexidine-resistant S. aureus strains; they also highlight the need for careful use of polyhexanide at low antiseptic concentrations.
Databáze: OpenAIRE
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